ECM550

QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), colorimetric

The CHEMICON Cell Invasion Assay Kit uses a 24-well plate, with 8 um pores, which provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.

• Synonym(s): Basement membrane cell invasion assay
• UNSPSC Code: 12352207
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• Quality Level: 100
• species reactivity: vertebrates
• manufacturer/tradename: Chemicon^®; QCM
• technique(s): cell based assay: suitable
• detection method: colorimetric
• shipped in: wet ice

Description

General description

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Introduction
Invasion through the extracellular matrix (ECM) is an important step in tumor metastasis. Cancer cells initiate invasion by adhering to and spreading along the blood vessel wall. Proteolytic enzymes, such as MMP collagenases, dissolve tiny holes in the sheath-like covering (basement membrane) surrounding the blood vessels to allow cancer cells to invade.

The CHEMICON Cell Invasion Assay Kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. The kit utilizes ECMatrix, a reconstituted basement membrane matrix of proteins derived from the Engelbreth Holm-Swarm (EHS) mouse tumor. We examined the kit′s performance using human fibrosarcoma (HT-1080) and non-invasive fibroblasts (NIH3T3).

The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.

The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.

Test Principle:

The CHEMICON Cell Invasion Assay is performed in an Invasion Chamber, a 24-well tissue culture plate with 12 cell culture inserts. The inserts contain an 8 μm pore size polycarbonate membrane, over which a thin layer of ECMatrixTM is dried. The ECM layer occludes the membrane pores, blocking non-invasive cells from migrating through. Invasive cells, on the other hand, migrate through the ECM layer and cling to the bottom of the polycarbonate membrane.

Application:

The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.

The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.

Application

Detection of migrating cells is via crystal violet stain.

Research Category
Cell Structure

Packaging

12 wells

Components

Invasion Chamber: (Part No. 70019) One 24-well plate with 12 ECMatrixTM layered cell culture inserts.

Cell Stain: (Part No. 20294) One 10 mL bottle.

Cotton Swabs: (Part No. 10202) 24 each.

Forceps: (Part No. 10203) One each.

Storage and Stability

Store kit materials at 2-8°C for up to 6 months. Do not freeze.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10-13 - German Storage Class 10 to 13
• WGK: WGK 1