07-081
Anti-phospho (Ser10)-acetyl (Lys14)-Histone H3 Antibody
Upstate®, from rabbit
Synonym(s):
H3K14me3S10P, Histone H3 (acetyl K14, phospho S10), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
About This Item
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
eukaryotes, mouse, human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer10), acetylation (Lys14)
Gene Information
human ... HIST1H3F(8968)
General description
Specificity
Immunogen
Application
Epigenetics & Nuclear Function
Histones
1:2000-1:10,000 dilutions of a previous lot detected phosphorylated and acetylated histone H3 in acid extracted proteins from serum starved mouse 10T1/2 cells treated with 50ng/ml EGF for 12.5 minutes. Western blotting of this lot to acid extracted proteins described above was preferentially competed by peptides in the following order: phospho-acetyl-H3 (7-20) > straight chain H3 (7-20) > phospho-H3 (7-20) or acetyl-H3 (7-20).
Peptide Inhibition Analysis: A 1:500 dilution from a representative lot was used with HeLa AE for peptide block analysis.
Chromatin Immunoprecipitation:
A previous lot of this antibody was reported by an independent laboratory to immunoprecipitate chromatin. (Cheung, P., 2000.)
Immunocytochemistry:
A previous lot of this antibody detected phosphorylated and acetylated histone H3 in EGF-stimulated cells; as reported by an independent laboratory. (Cheung, P., 2000.)
Quality
Western Blot Analysis:
1:500 dilution of this lot detected phospho acetyl Histone H3 on 10 μg of HeLa acid extract.
Target description
Physical form
Storage and Stability
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Acid-extracted proteins from serum-starved 10T1/2 cells.
Other Notes
Legal Information
Disclaimer
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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