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  • Mineralized gelatin methacrylate-based matrices induce osteogenic differentiation of human induced pluripotent stem cells.

Mineralized gelatin methacrylate-based matrices induce osteogenic differentiation of human induced pluripotent stem cells.

Acta biomaterialia (2014-08-26)
Heemin Kang, Yu-Ru V Shih, Yongsung Hwang, Cai Wen, Vikram Rao, Timothy Seo, Shyni Varghese
ABSTRACT

Human induced pluripotent stem cells (hiPSC) are a promising cell source with pluripotency and self-renewal properties. Design of simple and robust biomaterials with an innate ability to induce lineage-specificity of hiPSC is desirable to realize their application in regenerative medicine. In this study, the potential of biomaterials containing calcium phosphate minerals to induce osteogenic differentiation of hiPSC was investigated. hiPSC cultured using mineralized gelatin methacrylate-based matrices underwent osteogenic differentiation ex vivo, in both two-dimensional and three-dimensional cultures, in growth medium devoid of any osteogenic-inducing chemical components or growth factors. The findings that osteogenic differentiation of hiPSC can be achieved through biomaterial-based cues alone present new avenues for personalized regenerative medicine. Such biomaterials that could not only act as structural scaffolds, but could also provide tissue-specific functions such as directing stem cell differentiation commitment, have great potential in bone tissue engineering.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Gelatin methacryloyl, gel strength 300 g Bloom, degree of substitution 40%
Sigma-Aldrich
Gelatin methacryloyl, gel strength 300 g Bloom, 80% degree of substitution
Sigma-Aldrich
Gelatin methacryloyl, gel strength 90-110 g Bloom, degree of substitution 60%
Sigma-Aldrich
Gelatin methacryloyl, gel strength 300 g Bloom, degree of substitution 60%
Sigma-Aldrich
Gelatin methacryloyl, gel strength 170-195 g Bloom, degree of substitution: 60%
Sigma-Aldrich
Low endotoxin GelMA, gel strength 300 (Bloom), degree of substitution 80%, GelMA Type B
Sigma-Aldrich
Low endotoxin GelMA, mol wt 95 kDa, degree of substitution 60%
Sigma-Aldrich
Low endotoxin GelMA, bloom 300, Type A, degree of substitution 80%
Sigma-Aldrich
TissueFab® Discrete GelMA, 90-110 bloom, 50% degree of substitution
Sigma-Aldrich
TissueFab® Discrete GelMA, 300 bloom, 50% degree of substitution
Sigma-Aldrich
Low endotoxin GelMA solution, gel strength 300 (bloom), degree of substitution 80%, 0.2 μm, sterile-filtered, GelMA Type B
Sigma-Aldrich
TissueFab® Discrete GelMA, 300 bloom, 80% degree of substitution
Sigma-Aldrich
TissueFab® Discrete GelMA, 170-195 boom, 50% degree of substitution