Skip to Content
Merck
  • Engineering a Bioartificial Human Colon Model Through Decellularization and Recellularization.

Engineering a Bioartificial Human Colon Model Through Decellularization and Recellularization.

Methods in molecular biology (Clifton, N.J.) (2018-12-14)
Huanhuan Joyce Chen, Michael L Shuler
ABSTRACT

The tissue engineering method of decellularization and recellularization has been successfully used in a variety of regenerative medicine applications. The protocols used to de/recellularize various organs and tissues are largely different. Here we describe a method to effectively engineer a bioartificial colon by completely removing original cells from human intestinal tissues followed by repopulating the acellular tissue matrix with cell cultures. This method provides a novel approach for human intestinal regeneration and can be used to identify potential cancer driver genes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-CHGA antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-GPA33 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-TJP1 antibody produced in rabbit, Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-LAMB2 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Monoclonal Anti-PECAM1 antibody produced in mouse, clone 1D2-1A5, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-Fibronectin antibody produced in mouse, clone IST-4, ascites fluid
Sigma-Aldrich
Monoclonal Anti-Cytokeratin, pan (Mixture) antibody produced in mouse, clone C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2, ascites fluid