Skip to Content
Merck
  • NOGOB receptor-mediated RAS signaling pathway is a target for suppressing proliferating hemangioma.

NOGOB receptor-mediated RAS signaling pathway is a target for suppressing proliferating hemangioma.

JCI insight (2021-01-06)
Wenquan Hu, Zhong Liu, Valerie Salato, Paula E North, Joyce Bischoff, Suresh N Kumar, Zhi Fang, Sujith Rajan, M Mahmood Hussain, Qing R Miao
ABSTRACT

Infantile hemangioma is a vascular tumor characterized by the rapid growth of disorganized blood vessels followed by slow spontaneous involution. The underlying molecular mechanisms that regulate hemangioma proliferation and involution still are not well elucidated. Our previous studies reported that NOGOB receptor (NGBR), a transmembrane protein, is required for the translocation of prenylated RAS from the cytosol to the plasma membrane and promotes RAS activation. Here, we show that NGBR was highly expressed in the proliferating phase of infantile hemangioma, but its expression decreased in the involuting phase, suggesting that NGBR may have been involved in regulating the growth of proliferating hemangioma. Moreover, we demonstrate that NGBR knockdown in hemangioma stem cells (HemSCs) attenuated growth factor-stimulated RAS activation and diminished the migration and proliferation of HemSCs, which is consistent with the effects of RAS knockdown in HemSCs. In vivo differentiation assay further shows that NGBR knockdown inhibited blood vessel formation and adipocyte differentiation of HemSCs in immunodeficient mice. Our data suggest that NGBR served as a RAS modulator in controlling the growth and differentiation of HemSCs.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Caldesmon antibody produced in rabbit, affinity isolated antibody
Pricing and availability is not currently available.
Sigma-Aldrich
Monoclonal Anti-Actin, α-Smooth Muscle, clone 1A4, ascites fluid
Pricing and availability is not currently available.