GE29-0915-98

Superose^® 6 Increase

3.2/300, Cytiva 29-0915-98, 8.6 μm avg. part. size

• Synonym(s): Size exclusion chromatography, Superose 6 column
• UNSPSC Code: 41115700
• NACRES: SB.52

Properties

• material: glass column
• product line: Superose^®
• mol wt: 5000-5000000 Da (globular proteins)
• manufacturer/tradename: Cytiva 29-0915-98
• parameter: <0.03 mL/min flow rate (20% ethanol at low temp); <0.07 mL/min flow rate (20% ethanol at room temp); <0.07 mL/min flow rate (water at low temp); <0.15 mL/min flow rate (water at room temp); 3 Mpa (435 psi); 4-50 μL sample volume
• matrix: cross-linked agarose; polymeric
• matrix active group: agarose phase
• avg. part. size: 8.6 μm
• cleaning: 1-14
• working range: 3-12
• separation technique: size exclusion (SEC)

Description

General description

Superose^® 6 Increase 3.2/300 is a versatile, prepacked column for size exclusion chromatography in small-scale preparative purification (4 to 50 μl sample volume), as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000. When high resolution is required, sample amount is limited and low buffer consumption is important, this is the column of choice.

Features and Benefits

• Increased resolution compared with Superose 6, for higher purity and improved analysis results.
• Reduced runtime compared with Superose 6, for rapid results.
• Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
• Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

Suitability

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

Superdex is a registered trademark of Cytiva

Superose is a registered trademark of Cytiva

Safety Information

• Storage Class Code: 3 - Flammable liquids