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GE28-9042-67

illustra™ Plasmid Midi (25)

Cytiva 28-9042-67, pack of 1 × 25 pieces

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.52

packaging

pack of 1 × 25 pieces

manufacturer/tradename

Cytiva 28-9042-67

General description

PlasmidPrep Midi Flow Kit is a gravity flow plasmid prep kit designed for the purification of large yields of transfection-grade plasmid DNA that is proportional to the input sample volume.

The mechanism of adding a DNA plasmid into a mammalian cell is known as plasmid transfection, and for it to work, transfection-grade plasmid DNA is needed. To obtain efficient gene transfer by transfection, plasmid DNA can be complexed with lipid reagents for more efficient delivery into the cell′s nucleus, essential for subsequent protein expression of the gene of interest.

PlasmidPrep Midi Flow Kit can be used to process bacterial sample volumes of 25 to 50 mL for high copy number plasmid DNA and up to 500 mL for low copy number plasmid DNA. The kit consists of a high-performance, anion exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA.

PlasmidPrep Midi Flow Kit contains 250 fast-flow plasmid columns, resuspension, lysis, neutralization, wash, elution, and Tris-EDTA (TE) buffers, and a full protocol booklet with a detachable, quick reference protocol card.

Features and Benefits

  • Provides plasmid DNA with excellent purity, > 70% (strain-dependent) of supercoiled plasmid DNA
  • Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns
  • Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns.
  • Consistently produces pure plasmid DNA with low levels of endotoxin contamination, < 10 endotoxin units (EU) per μg of plasmid DNA

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

illustra is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

General Considerations for Plasmid DNA Preparation

This page discusses methods for plasmid DNA purification and the factors impacting resulting DNA purity and yield using illustra plasmidPrep Kits from Cytiva.

Plasmid DNA Preparation Troubleshooting

This page shows possible causes and solutions for problems that may occur during plasmid DNA preparation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service