SCM112

3dGRO^® Wnt3a Conditioned Media Supplement

• Synonym(s): Wnt3a Conditioned Media;Wnt3a CM;Wnt Conditioned Media
• UNSPSC Code: 41106514
• NACRES: NA.76

Properties

• packaging: 1 ea of 50 mL
• manufacturer/tradename: Millipore
• technique(s): cell culture | mammalian: suitable; cell culture | stem cell: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

Cell Line Description

3D organoid culture systems are increasingly employed as powerful tools for the study of human diseases. Wnt proteins hetero-dimerize Frizzled (Fzd) family receptors and their co-receptors Lrp5/6, triggering downstream signaling pathways including the canonical β-catenin resulting in expression of genes regulating cell development. Wnt3a is considered an essential protein in many organoid culture systems (intestinal, pancreatic, liver, colon etc.) because the proliferation of stem cells is governed by the Wnt signaling pathway. However, due to the high concentration requirement in organoid media, the use of the recombinant Wnt3a becomes prohibitively expensive. Additionally, recombinant Wnt3a proteins are often unstable and lack biological activity. The 3dGRO^® Wnt3a Conditioned Media Supplement is a potent and inexpensive alternative to purified recombinant Wnt3a protein for organoid cultures and is optimized for the highest level of Wnt3a expression to ensure consistency and performance.SourceThe 3dGRO^® Wnt3a Conditioned Media Supplement is generated from mouse L-M(TK-) cells transfected with a mouse Wnt-3A expression vector. The conditioned medium contains fetal bovine serum.

Application

• Appearance (color): clear, red liquid
• Endotoxin: 2 EU/mL
• Sterility Tested: No Growth/Pass
• Mycoplasma: Negative
• Media tested for function in supporting growth of human pancreatic organoids
• Media tested for biological activity using a Wnt3a luciferase reporter assay in HEK293 STF cells

Features and Benefits

Alternative stem cell media supplement to purified recombinant Wnt3a protein for organoid cultures.

Storage and Stability

Wnt3a Conditioned Medium should be stored at -20°C. When ready to use, thaw the supplement overnight at 2-8°C in the dark. Thawed Wnt3a Conditioned Medium is good for two weeks when stored at 2-8°C.

Legal Information

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable