06-935

Anti-Estrogen Receptor α Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): mouse (based on 100% sequence homology), rat (immunogen homology)
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_036821
• UniProt accession no.: P06211
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ESR1(2099) ; mouse ... Esr1(13982) ; rat ... Esr1(24890)

Description

General description

Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies.

Specificity

This antibody recognizes the C-terminus of Estrogen Receptor alpha.

Immunogen

Epitope: C-terminus.

KLH-conjugated linear peptide corresponding to the C-terminus of rat Estrogen Receptor alpha.

Application

• Tested applications
• Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution from a representative lot detected Estrogen Receptor in human stomach, human uterine cancer, human breast cancer and human cervical cancer tissue sections.
• Immunoprecipitation Analysis: 4 µg from a representative lot immunoprecipitated Estrogen Receptor in MCF-7 cell lysate.
• Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Estrogen Receptor in MCF-7 cells.
• Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. "

Anti-Estrogen Receptor , Cat. No. 06-935, is a rabbit polyclonal antibody that detects Estrogen Receptor and is tested for use in Western Blotting, Immunocytochemistry, Immunoprecipitation, and Immunohistochemistry (Paraffin).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Hormones & Receptors

Quality

Evaluated by Western Blotting in MCF-7 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Estrogen Receptor in MCF-7 cell lysate.

Target description

~58 kDa observed. UniProtKB/Swiss-Prot P06211 (ESR1_RAT) lists a molecular weight of 67 kDa A molecular weight between 52-58 kDA due to variant isoforms may be observed in some cell lysates (Al-Bader, M.D. (2006). Reprod Biol Endocrinol. 4:13.; Geisler, J.G. et al. (2002). Diabetes. 51(7):2158-2169.). Uncharacterized bands may be seen at a molecular weight of ~10 kDa, ~35 kDa, ~41 kDa, ~105 kDa, and ~220 kDa in some cell lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
MCF7 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable