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Key Documents

04-1667

Sigma-Aldrich

Rabbit-Anti-Human IgG4 Antibody, clone RM120

clone RM120, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

secondary antibodies

clone

RM120, monoclonal

species reactivity

human

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

shipped in

wet ice

target post-translational modification

unmodified

Specificity

This antibody reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.

Immunogen

Peptide corresponding to the hinge region of Human IgG4.

Application

Immunohistochemistry Analysis (IHC): 1 -10 μg/mL from a representative lot detected human IgG4 in human lymphoid tissue.

ELISA Analysis: The specificity and reactivity of a representative lot was confirmed by ELISA analyses.
Rabbit-Anti-Human IgG4 Antibody, clone RM120 is an antibody against Human IgG4 for use in Western Blotting, Immunohistochemistry, ELISA.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Quality

Evaluated by Western Blotting in human, mouse, rat, and goat IgG.

Western Blotting Analysis (WB): 2 μg/mL of this antibody detected human IgG in human, mouse, rat, and goat IgG.

Target description

(~150kDa, non-reduced) and heavy chain (~50kDa, reduced). No cross reactivity with other isotype of human IgG, and
mouse, rat, or goat IgG.

Physical form

Format: Purified
Protein A purified
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Possible Mechanism of Dysphania ambrosioides (L.) Mosyakin & Clemants Seed Extract Suppresses the Migration and Invasion of Human Hepatocellular Carcinoma Cells SMMC-7721.
Huang, et al.
Chemistry and Biodiversity, 20, e202200768-e202200768 (2023)

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