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  • Evaluating the efficiency of isotope transmission for improved panel design and a comparison of the detection sensitivities of mass cytometer instruments.

Evaluating the efficiency of isotope transmission for improved panel design and a comparison of the detection sensitivities of mass cytometer instruments.

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2015-02-24)
Sabine Tricot, Mickael Meyrand, Chiara Sammicheli, Jamila Elhmouzi-Younes, Aurélien Corneau, Sylvie Bertholet, Marie Malissen, Roger Le Grand, Sandra Nuti, Hervé Luche, Antonio Cosma
ABSTRACT

The recent introduction of mass cytometry, a technique coupling a cell introduction system generating a stream of single cells with mass spectrometry, has greatly increased the number of parameters that can be measured per single cell. As with all new technology there is a need for dissemination of standardization and quality control procedures. Here, we characterize variations in sensitivity observed across the mass range of a mass cytometer, using different lanthanide tags. We observed a five-fold difference in lanthanide detection over the mass range and demonstrated that each instrument has its own sensitivity pattern. Therefore, the selection of lanthanide combinations is a key step in the establishment of a staining panel for mass cytometry-based experiments, particularly for multicenter studies. We propose the sensitivity pattern as the basis for panel design, instrument standardization and future implementation of normalization algorithms.

MATERIALS
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