581302-U

Ascentis^® C18 (3 µm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

• Synonym(s): Ascentis RP18 HPLC Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Ascentis^® C18 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 2.1 mm
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L1 (Similar to Phenomenex Luna C18)
• product line: Ascentis^®
• feature: endcapped
• manufacturer/tradename: Ascentis^®
• packaging: 1 ea of
• extent of labeling: 25% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 15 cm × 2.1 mm
• surface area: 450 m²/g
• surface coverage: 3.7 μmol/m²
• impurities: <5 ppm metals
• matrix: fully porous particle; silica gel high purity, spherical
• matrix active group: C18 (octadecyl) phase
• particle size: 3 μm
• pore size: 100 Å
• operating pH range: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Application

• A Comparative Study on Analysis of Ginsenosides in American Ginseng Root Residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS/MS.: This paper compares methodologies involving the Ascentis^® C18 HPLC Column for analyzing ginsenosides, showcasing the column′s utility in herbal medicine research (Hsu et al., 2022).


• Validation of a Rapid and Easy-to-Apply Method to Simultaneously Quantify Co-Loaded Dexamethasone and Melatonin PLGA Microspheres by HPLC-UV: Encapsulation Efficiency and In Vitro Release.: Demonstrates the use of the Ascentis^® C18 HPLC Column in developing a validated HPLC-UV method for analyzing complex drug delivery systems, highlighting the column′s versatility (Brugnera et al., 2022).


• Simultaneous determination of ivermectin, clorsulon and their related substances in an injectable finished product by a stability-indicating RP-HPLC method.: Utilizes the Ascentis^® C18 HPLC Column to ensure the stability and quality of veterinary pharmaceuticals, underlining the column′s application in regulatory compliance (Padivitage et al., 2022).

Features and Benefits

• Excellent retention
• Symmetric peak shape
• High reproducibility
• Complete LC-MS compatibility

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable