567513-U
Discovery® HS F5 (5 µm) HPLC Columns
L × I.D. 5 cm × 4.6 mm, HPLC Column
Synonym(s):
Discovery Pentafluorophenyl PFP HPLC Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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product name
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
material
stainless steel column
Agency
suitable for USP L43
product line
Discovery®
feature
endcapped
manufacturer/tradename
Discovery®
packaging
1 ea of
extent of labeling
12% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
5 cm × 4.6 mm
surface area
300 m2/g
surface coverage
4 μmol/m2
impurities
<10 ppm metals
matrix
silica gel, high purity, spherical particle platform
fully porous particle
matrix active group
PFP (pentafluorophenyl) phase
particle size
5 μm
pore size
120 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
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General description
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
Application
- Optimization and validation of a bioanalytical HPLC-UV technique for simultaneous determination of underivatized phenylalanine and tyrosine in the blood for phenylketonuria diagnosis and monitoring.: This study utilizes the Discovery® HS F5 HPLC Column to develop an HPLC-UV method for the simultaneous determination of phenylalanine and tyrosine in blood, significantly advancing the diagnostic and monitoring capabilities for phenylketonuria (Rabti et al., 2024).
- A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicities.: Demonstrates the application of the Discovery® HS F5 HPLC Column in optimizing sample preparation techniques for the detection of pharmaceuticals and personal care products in environmental samples, enhancing the analysis of diverse hydrophilic compounds (Portillo-Castillo et al., 2021).
- "Heart-cut" bidimensional achiral-chiral liquid chromatography applied to the evaluation of stereoselective metabolism, in vivo biological activity and brain response to chiral drug candidates targeting the central nervous system.: Utilizes the Discovery® HS F5 HPLC Column in a novel bidimensional chromatographic setup to study the stereoselective metabolism of CNS-targeting chiral drugs, contributing to a deeper understanding of their pharmacological profiles (Battisti et al., 2016).
- HPLC method development, validation, and impurity characterization of a potent antitumor indenoisoquinoline, LMP776 (NSC 725776).: This research employs the Discovery® HS F5 HPLC Column for developing an HPLC method to identify and characterize impurities in the antitumor drug LMP776, ensuring drug purity and compliance with regulatory standards (Wang et al., 2016).
- Development studies on determination of preservatives decomposition products.: Showcases the application of the Discovery® HS F5 HPLC Column in the analysis of decomposition products of preservatives, enhancing the safety assessments of food and pharmaceutical products (Zabrzewska et al., 2014).
Features and Benefits
- Unique selectivity
- Similar retention to C18 (sometimes requires stronger mobile phase)
- Excellent peak shape
- Stable, low-bleed LC-MS separations
- Scalable separations from 3 to 10μm particle sizes
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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C Desmortreux et al.
Journal of chromatography. A, 1216(42), 7088-7095 (2009-09-15)
Separation of furocoumarins has become of a great interest for cosmetic industry and human health, since the recent directive of the European Union. Furocoumarins are a class of compounds presenting varied substituents linked mainly in two positions to an identical
Lucie Havlíková et al.
Talanta, 76(3), 597-601 (2008-07-01)
A pentafluorophenylpropyl (PFPP) stationary phase was for the first time tested for the simultaneous determination of triamcinolone acetonide, its degradation product triamcinolone and two preservatives, methylparaben, and propylparaben. A new simple isocratic reversed phase HPLC method with UV detection, using
Hiroyuki Kataoka et al.
Analytical and bioanalytical chemistry, 405(1), 331-340 (2012-10-16)
We have developed a simple and sensitive method for the simultaneous determination of testosterone (TES), cortisol (CRT), and dehydroepiandrosterone (DHEA) in saliva by automated online in-tube solid-phase microextraction (SPME) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a Discovery HS
Milan Nobilis et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(32), 3845-3852 (2011-11-22)
New bioanalytical SPE-HPLC-PDA-FL method for the determination of the neuroleptic drug tiapride and its N-desethyl metabolite was developed, validated and applied to xenobiochemical and pharmacokinetic studies in humans and animals. The sample preparation process involved solid-phase extraction of diluted plasma
Jian Mao et al.
Se pu = Chinese journal of chromatography, 27(2), 216-219 (2009-07-25)
A simple method was developed for the analysis of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline (6-OH-MTHbetaC), 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) in rat brain by high performance liquid chromatography with electrochemical detection (HPLC-ECD). The separation of the sample was performed on a Discovery
Chromatograms
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