M3795
IgM, Kappa from murine myeloma
clone TEPC 183, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Mouse IgM-κ
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
clone
TEPC 183, monoclonal
Assay
≥90% (HPLC)
form
buffered aqueous solution
concentration
2.0-2.2 mg/mL
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Determined by the absorbance at 280 nm (E1%/280 = 11.8). Each vial contains at least 1 mg of purified myeloma protein.
IgM antibodies are present as pentamers in the serum and are produced in response to antigens IgM, κ from murine myeloma is produced by TEPC 183 tumor line introduced subcutaneously in BALB/c mice.
IgM is a highly conserved antibody in vertebrates and is expressed early during immune response. It is secreted by peritoneal B cells.
Specificity
The TEPC 183 tumor line is pristine-induced plasmacytoma-originated and carried subcutaneously in BALB/c mice. The hapten binding specificity of the TEPC 183 line has not been determined. It specifically recognizes mouse IgM. Identity and purity of the immunoglobulin is established by immunoelectrophoresis.
Application
IgM, Kappa from murine myeloma has been used in:
- sandwich enzyme linked immunosorbent assay (ELISA)
- flow cytometry
- dot-immunobinding assay
Biochem/physiol Actions
IgM plays a key role in the engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules. IgM has sites for N-linked glycosylation, with sugars namely, mannose, galactose, N-acetyl glucosamine and sialic acid. IgM linked agarose resins have been tested for efficient conjugate binding.
Physical form
Solution in 0.05 M Tris, 0.5 M sodium chloride, pH 8.0, containing 0.02% sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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