- Novel bacterial molybdenum and tungsten enzymes: three-dimensional structure, spectroscopy, and reaction mechanism.
Novel bacterial molybdenum and tungsten enzymes: three-dimensional structure, spectroscopy, and reaction mechanism.
Biological chemistry (2005-10-13)
Matthias Boll, Bernhard Schink, Albrecht Messerschmidt, Peter M H Kroneck
PMID16218872
ABSTRACT
The molybdenum enzymes 4-hydroxybenzoyl-CoA reductase and pyrogallol-phloroglucinol transhydroxylase and the tungsten enzyme acetylene hydratase catalyze reductive dehydroxylation reactions, i.e., transhydroxylation between phenolic residues and the addition of water to a triple bond. Such activities are unusual for this class of enzymes, which carry either a mononuclear Mo or W center. Crystallization and subsequent structural analysis by high-resolution X-ray crystallography has helped to resolve the reaction centers of these enzymes to a degree that allows us to understand the interaction of the enzyme and the respective substrate(s) in detail, and to develop a concept for the respective reaction mechanism, at least in two cases.
MATERIALS
Product Number
Brand
Product Description
Tungsten, insulated wire, 5m, conductor diameter 0.075mm, insulation thickness 0.02mm, PTFE (polytetrafluoroethylene) insulation, 99.9%
Tungsten, insulated wire, 10m, conductor diameter 0.05mm, insulation thickness 0.008mm, polyimide insulation, 99.9%
Tungsten, microfoil, disks, 10mm, thinness 0.25μm, specific density 474μg/cm2, permanent mylar 3.5μm support, 99.95%
Tungsten, insulated wire, 0.2m, conductor diameter 0.015mm, insulation thickness 0.002mm, polyimide insulation, 99.9%
Tungsten, foil, 5m coil, thickness 0.025mm, coil width 1mm, as rolled, 99.95%
Tungsten, insulated wire, 20m, conductor diameter 0.05mm, insulation thickness 0.008mm, polyimide insulation, 99.9%
Tungsten, foil, not light tested, 50x50mm, thickness 0.004mm, 99.95%
Tungsten, foil, light tested, 150x150mm, thickness 0.05mm, as rolled, 99.95%
Tungsten, lump, 200g, max. size 5mm
Tungsten, insulated wire, 2m, conductor diameter 0.075mm, insulation thickness 0.02mm, PTFE (polytetrafluoroethylene) insulation, 99.9%
Tungsten, rod, 200mm, diameter 6.35mm, centerless ground, 99.95%
Tungsten, foil, light tested, 50x50mm, thickness 0.075mm, as rolled, 99.95%
Tungsten, foil, not light tested, 25x25mm, thickness 0.004mm, 99.95%
Tungsten, insulated wire, 2m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyimide insulation, 99.9%
Tungsten, foil, 30mm disks, thickness 1.0mm, as rolled, 99.95%
Tungsten, foil, light tested, 150x150mm, thickness 0.075mm, as rolled, 99.95%
Tungsten, lump, 50g, max. size 5mm
Tungsten, insulated wire, 1m, conductor diameter 0.015mm, insulation thickness 0.002mm, polyimide insulation, 99.9%
Tungsten, microfoil, disks, 10mm, thinness 0.05μm, specific density 96μg/cm2, permanent mylar 3.5μm support, 99.95%
Tungsten, insulated wire, 2m, conductor diameter 0.0125mm, insulation thickness 0.0035mm, polyimide insulation, 99.9%
Tungsten, foil, light tested, 150x150mm, thickness 0.025mm, as rolled, 99.95%
Tungsten, microfoil, disks, 10mm, thinness 0.1μm, specific density 182μg/cm2, permanent mylar 3.5μm support, 99.95%
Tungsten, wire reel, 0.5m, diameter 1.0mm, clean, 99.95%
Tungsten, insulated wire, 2m, conductor diameter 0.015mm, insulation thickness 0.002mm, polyimide insulation, 99.9%
Tungsten, foil, not light tested, 50x50mm, thickness 0.007mm, 99.95%
Tungsten, foil, not light tested, 25x25mm, thickness 0.006mm, 99.95%
Tungsten, rod, 100mm, diameter 5.0mm, centerless ground, 99.95%
Tungsten, mesh, 100x100mm, nominal aperture 0.2mm, wire diameter 0.05mm, 100 wires/inch, open area 64%, plain weave mesh, 99.95%