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H2519

Sigma-Aldrich

Heparinase I from Flavobacterium heparinum

Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA)

Synonym(s):

Heparin lyase I, Heparinase

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About This Item

CAS Number:
9025-39-2
Enzyme Commission number:
4.2.2.7 (BRENDA, IUBMB)
MDL number:
MFCD00131317
UNSPSC Code:
12352204
NACRES:
NA.54

conjugate

conjugate (Glucosaminoglycan)

Quality Level

200

form

lyophilized solid

specific activity

≥200 units/mg protein (enzyme + BSA)

mol wt

42.8 kDa

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

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Application

Heparinase I from Flavobacterium heparinum has been used:
  • in the digestion of plasma samples
  • to study the effect of medium, washing and sonication on the infectivity of Chlamydia pneumonia
  • in enzymatic removal of heparan sulfate from the surface of human rhabdomyosarcoma cells

Biochem/physiol Actions

Heparinase I from Flavobacterium heparinum acts by cleaving the α-glycoside linkage at AT III binding site in heparin. This results in small oligosaccharide fragments . Heparinase I neutralizes the protein′s action of anticoagulation. Heparinase I possesses a half-life of 15-18 minutes.

Unit Definition

One unit (Sigma unit) will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Methodological aspects affecting the infectivity of Chlamydia pneumoniae in cell cultures in vitro
Yan Y, et al.
Journal of Microbiological Methods, 61(1), 127-130 (2005)
HeparinHeparin - A Century of Progress, 273-273 (2012)
Wataru Hashimoto et al.
Biochemistry, 53(4), 777-786 (2014-01-21)
Pedobacter heparinus (formerly known as Flavobacterium heparinum) is a typical glycosaminoglycan-degrading bacterium that produces three heparin lyases, Hep I, Hep II, and Hep III, which act on heparins with 1,4-glycoside bonds between uronate and amino sugar residues. Being different from
Cardiopulmonary Bypass: Principles and Practice, 509-509 (2008)
M E Casey et al.
Anaesthesia, 68(1), 91-96 (2012-10-16)
Paediatric patients who require anticoagulation with therapeutic doses of low-molecular weight heparin are at risk of having a residual anticoagulant effect at the time of surgery, even if managed according to current peri-operative guidelines. Testing for residual effect is not
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