MAB1987Z

Anti-Integrin β1 Antibody, clone P4C10, azide free

clone P4C10, Chemicon^®, from mouse

• Synonym(s): CD29, MAB1987
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: P4C10, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2a
• NCBI accession no.: NM_002211.2; NM_033666.1; NM_033667.1; NM_033668.1; NM_033669.1; NM_133376.1
• UniProt accession no.: P05556
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ITGB1(3688)

Description

General description

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

Specificity

Reacts with the beta-1 subunit of the integrin protein family. Appears to be human specific.Unlike many beta-1 monoclonals 4C10′s epitope is not trypsin sensitive.

Application

Anti-Integrin β1 Antibody, clone P4C10, azide free detects level of Integrin β1 & has been published & validated for use in ELISA, IC, IP, FUNC & WB.

Inhibits attachment of UCLA-P3 cells to collagen type I (Wayner, 1991). Inhibits endodermal and keratinocytes to collagen, fibronectin and laminin. It also inhibits cell to cell adhesion. Typical dilution ranges are 1:50-1:500.

Immunoflourescence

Immunoblotting

Immunoprecipitation

ELISA

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin β1 in HeLa cells.

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Target description

88 KDa

Linkage

Replaces: 04-1109

Physical form

Format: Purified

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.

Protein A purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Human Tonsil, human skin, human lung, liver, and skeletal muscle tissues

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable