365253

Gö 6976

InSolution, ≥95%, Inhibitor of protein kinase C

• Synonym(s): InSolution Gö 6976, Go 6976, Solution, 12-(2-Cyanoethyl)-6,7,12,13-tetrahydro-13-methyl-5-oxo-5H-indolo(2,3-a)pyrrolo(3,4-c)-carbazole
• Empirical Formula (Hill Notation): C₂₄H₁₈N₄O
• CAS Number: 136194-77-9
• Molecular Weight: 378.43
• MDL number: MFCD00236434
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• solubility: DMSO: 5 mg/mL
• shipped in: wet ice
• storage temp.: −20°C
• InChI: 1S/C24H18N4O/c1-27-17-9-4-2-7-14(17)20-21-16(13-26-24(21)29)19-15-8-3-5-10-18(15)28(12-6-11-25)23(19)22(20)27/h2-5,7-10H,6,12-13H2,1H3,(H,26,29)
• InChI key: VWVYILCFSYNJHF-UHFFFAOYSA-N

Description

General description

Inhibitor of protein kinase C (PKC; IC₅₀ = 7.9 nM for rat brain). Selectively inhibits Ca²⁺-dependent PKC_α-isozyme (IC₅₀ = 2.3 nM) and PKC_βI (IC₅₀ = 6.2 nM). Does not affect the kinase activity of the Ca²⁺-independent PKC δ-, ε-, and ζ-isoenzymes even at micromolar levels. A potent antagonist of HIV-1 induction.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
PKC

Product competes with ATP.

Reversible: yes

Target IC₅₀: 7.9 nM, 2.3 nM, and 6.2 nM, for rat brain PKC, Ca2+-dependent PKC α-isozyme, and PKCβI, respectively

Packaging

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Physical form

A 500 µg/ml solution of Gö 6976 (Cat. No. 365250) in anhydrous DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Gschwendt, M., et al. 1996. FEBS Lett.392, 77.
Wenzel-Seifert, K., et al. 1994. Biochem. Biophys. Res. Commun.200, 1536.
Martiny-Baron, G., et al. 1993. J. Biol. Chem.268, 9194.
Qatsha, K.A., et al. 1993. Proc. Natl. Acad. Sci. USA90, 674.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Flash Point(C): 87 °C - closed cup - (Dimethylsulfoxide)