HPLC (Size Exclusion) Analysis of Proteins on Zenix^®-C SEC-300, Effect of DTT and EDTA

column

Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777092)

mobile phase

(A) 150 mM sodium phosphate buffer, pH 7.0 (B) 150 mM sodium phosphate buffer, pH 7.0 with 2 mM DTT, 0.1 mM EDTA (C) 150 mM sodium phosphate buffer, pH 7.0 with 2 mM DTT, 0.1 mM EDTA

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 280 nm

injection

10 μL

sample

proteins