HPLC Analysis of cis/trans-Dothiepin Enantiomers on Astec^® CYCLOBOND I 2000 AC

column

CYCLOBOND I 2000 AC, 25 cm x 4.6 mm I.D., 5 μm particles (20124AST)

mobile phase

[A] 1.0% triethylamine acetate, pH 4.1; [B] acetonitrile; (85:15, A:B)

flow rate

1 mL/min

column temp.

22 °C

detector

UV, 254 nm

injection

2 μL

sample

1 mg/mL in methanol
peak 1: 9.48 min.
peak 2: 12.48 min.