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Merck

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
RÉSUMÉ

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATÉRIAUX
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Description du produit

Sigma-Aldrich
Méthanol, suitable for HPLC, ≥99.9%
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Acétone, ACS reagent, ≥99.5%
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Tetrahydrofurane, inhibitor-free, suitable for HPLC, ≥99.9%
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Méthanol, ACS reagent, ≥99.8%
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Hydroxyde d′ammonium solution, ACS reagent, 28.0-30.0% NH3 basis
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Acétone, suitable for HPLC, ≥99.9%
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N,N-Diméthylformamide, ACS reagent, ≥99.8%
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N,N-Diméthylformamide, suitable for HPLC, ≥99.9%
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Acétone, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
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Toluène, ACS reagent, ≥99.5%
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Chloroforme, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
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Tetrahydrofurane, contains 250 ppm BHT as inhibitor, ACS reagent, ≥99.0%
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Éther diéthylique, anhydrous, ACS reagent, ≥99.0%, contains BHT as inhibitor
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Pyridine, ACS reagent, ≥99.0%
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Chloroforme, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer
Sigma-Aldrich
Méthanol, HPLC Plus, ≥99.9%
Sigma-Aldrich
Éther diéthylique, suitable for HPLC, ≥99.9%, inhibitor-free
Sigma-Aldrich
Chloroforme, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
Éther diéthylique, ACS reagent, anhydrous, ≥99.0%, contains BHT as inhibitor
Sigma-Aldrich
Hydroxyde d′ammonium solution, 28% NH3 in H2O, ≥99.99% trace metals basis
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2-mercaptoéthanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
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2-mercaptoéthanol, ≥99.0%
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Chloroforme, contains ethanol as stabilizer, ACS reagent, ≥99.8%
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Formaldéhyde solution, for molecular biology, 36.5-38% in H2O
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Chlorure de sodium, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
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Pyridine, suitable for HPLC, ≥99.9%
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Méthanol, suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%
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Chlorure de calcium solution, BioUltra, for molecular biology, ~1 M in H2O
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Alcool benzylique, ReagentPlus®, ≥99%
SAFC
Formaldéhyde solution, contains 10-15% methanol as stabilizer, 37 wt. % in H2O