Accéder au contenu
Merck
  • Thyroid hormone-regulated Wnt5a/Ror2 signaling is essential for dedifferentiation of larval epithelial cells into adult stem cells in the Xenopus laevis intestine.

Thyroid hormone-regulated Wnt5a/Ror2 signaling is essential for dedifferentiation of larval epithelial cells into adult stem cells in the Xenopus laevis intestine.

PloS one (2014-09-12)
Atsuko Ishizuya-Oka, Mitsuko Kajita, Takashi Hasebe
RÉSUMÉ

Amphibian intestinal remodeling, where thyroid hormone (T3) induces some larval epithelial cells to become adult stem cells analogous to the mammalian intestinal ones, serves as a unique model for studying how the adult stem cells are formed. To clarify its molecular mechanisms, we here investigated roles of non-canonical Wnt signaling in the larval-to-adult intestinal remodeling during Xenopus laevis metamorphosis. Our quantitative RT-PCR (qRT-PCR) and immunohistochemical analyses indicated that the expressions of Wnt5a and its receptors, frizzled 2 (Fzd2) and receptor tyrosine kinase-like orphan receptor 2 (Ror2) are up-regulated by T3 and are spatiotemporally correlated with adult epithelial development in the X. laevis intestine. Notably, changes in morphology of larval absorptive epithelial cells expressing Ror2 coincide well with formation of the adult stem cells during metamorphosis. In addition, by using organ cultures of the tadpole intestine, we have experimentally shown that addition of exogenous Wnt5a protein to the culture medium causes morphological changes in the larval epithelium expressing Ror2 even in the absence of T3. In contrast, in the presence of T3 where the adult stem cells are formed in vitro, inhibition of endogenous Wnt5a by an anti-Wnt5a antibody suppressed the epithelial morphological changes, leading to the failure of stem cell formation. Our findings strongly suggest that the adult stem cells originate from the larval absorptive cells expressing Ror2, which require Wnt5a/Ror2 signaling for their dedifferentiation accompanied by changes in cell morphology.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
HEPES, ≥99.5% (titration)
Sigma-Aldrich
HEPES, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
Sigma-Aldrich
Chlorure de magnésium, anhydrous, ≥98%
Sigma-Aldrich
Chlorure de magnésium solution, for molecular biology, 1.00 M±0.01 M
Sigma-Aldrich
Hydrocortisone, BioReagent, suitable for cell culture
Sigma-Aldrich
Hydrocortisone, γ-irradiated, powder, BioXtra, suitable for cell culture
Sigma-Aldrich
HEPES, BioUltra, for molecular biology, ≥99.5% (T)
Sigma-Aldrich
HEPES solution, 1 M in H2O
Sigma-Aldrich
Hydrocortisone, ≥98% (HPLC)
SAFC
HEPES
Sigma-Aldrich
Chlorure de magnésium, powder, <200 μm
Sigma-Aldrich
Chlorure de magnésium solution, BioUltra, for molecular biology, 2 M in H2O
Sigma-Aldrich
Chlorure de magnésium, BioReagent, suitable for insect cell culture, ≥97.0%
Sigma-Aldrich
HEPES, BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)
SAFC
HEPES
Sigma-Aldrich
Chlorure de magnésium solution, PCR Reagent, 25 mM MgCI2 solution for PCR
Sigma-Aldrich
HEPES, BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)
Sigma-Aldrich
Chlorure de magnésium solution, BioUltra, for molecular biology, ~1 M in H2O
Sigma-Aldrich
Chlorure de magnésium, AnhydroBeads, −10 mesh, 99.9% trace metals basis
USP
Hydrocortisone, United States Pharmacopeia (USP) Reference Standard
Supelco
Hydrocortisone, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Chlorure de magnésium, AnhydroBeads, −10 mesh, 99.99% trace metals basis
Sigma-Aldrich
Chlorure de magnésium solution, 0.1 M
Sigma-Aldrich
Hydrocortisone, meets USP testing specifications
Sigma-Aldrich
HEPES, anhydrous, free-flowing, Redi-Dri, ≥99.5%
Supelco
HEPES, Pharmaceutical Secondary Standard; Certified Reference Material
Hydrocortisone, European Pharmacopoeia (EP) Reference Standard
Hydrocortisone, British Pharmacopoeia (BP) Assay Standard
Sigma-Aldrich
Chlorure de magnésium solution, BioUltra, for molecular biology, ~0.025 M in H2O
Sigma-Aldrich
MISSION® esiRNA, targeting human ROR2