- In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids.
In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids.
Angewandte Chemie (International ed. in English) (2012-10-12)
Erkin Kuru, H Velocity Hughes, Pamela J Brown, Edward Hall, Srinivas Tekkam, Felipe Cava, Miguel A de Pedro, Yves V Brun, Michael S VanNieuwenhze
PMID23055266
RÉSUMÉ
Tracking a bug's life: Peptidoglycan (PG) of diverse bacteria is labeled by exploiting the tolerance of cells for incorporating different non-natural D-amino acids. These nontoxic D-amino acids preferably label the sites of active PG synthesis, thereby enabling fine spatiotemporal tracking of cell-wall dynamics in phylogenetically and morphologically diverse bacteria. HCC = 7-hydroxycoumarin, NBD = 7-nitrobenzofurazan, TAMRA = carboxytetramethylrhodamine.
MATÉRIAUX
Référence du produit
Marque
Description du produit