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Merck

Single conical track-etched nanopore for a free-label detection of OSCS contaminants in heparin.

Biosensors & bioelectronics (2019-05-18)
Tianji Ma, Emmanuel Balanzat, Jean-Marc Janot, Sébastien Balme
RÉSUMÉ

The heparin contamination by oversulfated chondroitin (OSCS) was at the origin of one major sanitary problem of last decade. Here we propose a novel strategy to detect OSCS from heparin solution based on conical nanopore functionalized with poly-L-lysine deposition to ensure its re-usability. This sensor is an excellent to detect low heparin concentration (from 25 ng/ml to 3 μg/ml) using the modification of ionic current rectification. It also allows following the kinetic of heparin degradation by heparinase with a good correlation with results obtained by classical methods. The sensor is sensitive to the inhibition of heparinase by OSCS until a concentration of 200 pg/ml representing 0.01% in weight in a heparin. This resolution is one order of magnitude lower than the one obtained by chromatography. For the first time, it was reached without fluorescence labeling.

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Description du produit

Sigma-Aldrich
Heparinase I and III Blend from Flavobacterium heparinum, lyophilized powder, stabilized with ∼ 25% (w/w) bovine serum albumin, ≥200 unit/mg protein (enzyme + BSA)
Sigma-Aldrich
Heparinase II from Flavobacterium heparinum, Lyophilized powder stabilized with approx. 25% bovine serum albumin, lyophilized powder, ≥100 units/mg protein (enzyme + BSA)