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  • Structural characterization and in vitro hepatoprotective activity of polysaccharide from pine nut (Pinus koraiensis Sieb. et Zucc.).

Structural characterization and in vitro hepatoprotective activity of polysaccharide from pine nut (Pinus koraiensis Sieb. et Zucc.).

Carbohydrate polymers (2019-08-21)
Hang Qu, Xin Gao, Hai-Tian Zhao, Zhen-Yu Wang, Juan-Juan Yi
RÉSUMÉ

A new purified polysaccharide (PNP40c-1) with a molecular weight of 2.06 × 105 Da was obtained from pine nuts (Pinus koraiensis Sieb. et Zucc.). Structural analysis indicated that PNP40c-1 is a homogeneous heteropolysaccharide composed of arabinose, rhamnose and glucose in a molar ratio of 2.98:1.00:0.52. The major backbone consisted of →3,4)-α-l-Arap(1→, →4)-α-l-Arap3Me(1→, →3)-α-l-Rhap(1→ and →6)-β-d-Glcp(1→, and the side chain is β-d-Glcp-(1→ linked at C4-position of →3,4)-α-l-Arap(1→. In addition, the hepatoprotective effect of PNP40c-1 was investigated by human hepatocyte cell line L02 treated with carbon tetrachloride (CCl4). Results suggested that PNP40c-1 could protect L02 cells from CCl4-induced damage by enhancing the activities of antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), increasing the level of non-enzymatic antioxidant glutathione (GSH), suppressing lipid perioxidation and further reducing the leakage of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Hence, PNP40c-1 could be a promising functional food to serve as hepatoprotective agent.