ZRB2974

Anti-cPLA2 Antibody, clone 2F4 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Cytosolic phospholipase A2, Phospholipase A2 group IVA
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 2F4, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 85.24 kDa; observed mol wt ~100 kDa
• Produit purifié par: using Protein A
• Espèces réactives: human
• Conditionnement: antibody small pack of 25
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• Technique(s): affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: Internal
• Numéro d'accès Protein ID: NP_077734
• Numéro d'accès UniProt: P47712
• Autre catégorie plus écologique: Aligned
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Informations sur le gène: human ... PLA2G4A(5321)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 2F4 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects cytosolic phospholipase A2 (cPLA2). It targets an epitope within 23 amino acids from the internal region.

Immunogène

KLH-conjugated linear peptide corresponding to 23 amino acids from the internal region of human cytosolic Phospholipase A2 (cPLA2).

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected cPLA2 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected cPLA2 in lysates from A549 cells and Human testis tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected cPLA2 in Human kidney and 1:100 dilution in Human testis tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound cPLA2 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Cytosolic phospholipase A2 (UniProt: P47712; also known as cPLA2, Phospholipase A2 group IVA) is encoded by the PLA2G4A (also known as CPLA2, PLA2G4) gene (Gene ID: 5321) in human. cPLA2 is a calcium-dependent phospholipase with lysophospholipase activities. However, it also displays calcium-independent phospholipase A2 and lysophospholipase activities in the presence of phosphoinositides. It preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids. Its expression is observed in various cells and tissues, including macrophages, neutrophils, fibroblasts, and lung endothelium. It is detected in cytoplasm, nucleus, and Golgi apparatus membranes. It plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It also plays a role in embryo implantation and parturition through its ability to trigger prostanoid production. It is reported to selectively hydrolyze sn-2 arachidonoyl group from membrane phospholipids, thereby providing the precursor for eicosanoid biosynthesis via the cyclooxygenase pathway. cPLA2 undergoes phosphorylation at serine 505 and serine 727 by MAP kinases in response to mitogenic stimuli. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: (Ref.: Six, DA., and Dennis EA. (2003). J. Biol. Chem. 278(26); 23842-23850; Hanel, AM., and Gelb, MH. (1995). Biochemistry. 34(24); 7807-7818; Lin, LL., et al. (1993). Cell. 72(2); 269-278).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable