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SRP6483

Sigma-Aldrich

Carbonic Anhydrase 9/CA9 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonyme(s) :

CA-IX, CAIX, G250, MN, P54/58N, pMW

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About This Item

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in HEK 293 cells

Étiquette/Marqueur

6-His tagged (C-terminus)

Pureté

≥95% (SDS-PAGE)

Forme

lyophilized

Poids mol.

calculated mol wt 41.2 kDa
observed mol wt 53-54 kDa (DTT-reduced. Protein migrates due to glycosylation. Gln 38 is the predicted N-terminal.)

Conditionnement

pkg of 10 μg

Impuretés

<1 EU/μg endotoxin (LAL test)

Numéro d'accès UniProt

Q16790

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... CA9(768)

Description générale

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. There are at least five distinct CA families (α, β, γ, δ and ε). Carbonic anhydrase 9 (CA9 / CAIX), also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the α-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia.

Forme physique

Lyophilized from 0.22 μm filtered solution in 20 mM MES and 100 mM NaCl, pH 6.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Tayfun Arslan et al.
Dalton transactions (Cambridge, England : 2003), 49(1), 203-209 (2019-12-07)
In this study, phthalocyanine precursors (5 and 9) and 1,2,3-triazole-substituted metal-free and metallo phthalocyanines (9a-c) were designed and synthesized for the first time and evaluated in vitro for key molecular targets. The structures of the novel compounds were characterized via
Javier Quero et al.
Biomedicines, 10(6) (2022-06-25)
Two new families of dithiocarbamate gold(I) complexes derived from benzenesulfonamide with phosphine or carbene as ancillary ligands have been synthesized and characterized. In the screening of their in vitro activity on human colon carcinoma cells (Caco-2), we found that the

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