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H5000

Sigma-Aldrich

Hexokinase from Saccharomyces cerevisiae

Type III, lyophilized powder, ≥25 units/mg protein (biuret)

Synonyme(s) :

ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast

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About This Item

Numéro CAS:
9001-51-8
Numéro de classification (Commission des enzymes):
2.7.1.1 (BRENDA, IUBMB)
Numéro CE :
232-611-5
Numéro MDL:
MFCD00131321
Code UNSPSC :
12352204
eCl@ss :
32160410

Type

Type III

Forme

lyophilized powder

Activité spécifique

≥25 units/mg protein (biuret)

Poids mol.

dimer 110 kDa

Numéro d'accès UniProt

P04806
P04807

Activité étrangère

glucose-6-phosphate dehydrogenase and phosphoglucose isomerase ≤10%

Température de stockage

−20°C

Informations sur le gène

bakers yeast ... HXK1(850614) , HXK2(852639)

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Actions biochimiques/physiologiques

Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.

The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose KM: 0.33 mM
D-glucose KM: 0.12 mM
D-mannose KM: 0.05 mM

Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.

Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.

Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)

Définition de l'unité

One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C, unless otherwise indicated below.

Forme physique

Lyophilized powder containing approx. 15% sodium citrate

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Seok-Hyung Kim et al.
PLoS genetics, 9(6), e1003563-e1003563 (2013-06-21)
Multiple Acyl-CoA Dehydrogenase Deficiency (MADD) is a severe mitochondrial disorder featuring multi-organ dysfunction. Mutations in either the ETFA, ETFB, and ETFDH genes can cause MADD but very little is known about disease specific mechanisms due to a paucity of animal
Charles Betz et al.
The Journal of cell biology, 203(4), 563-574 (2014-01-05)
Target of rapamycin (TOR) forms two conserved, structurally distinct kinase complexes termed TOR complex 1 (TORC1) and TORC2. Each complex phosphorylates a different set of substrates to regulate cell growth. In mammals, mTOR is stimulated by nutrients and growth factors
Jean C-Y Tien et al.
Cancer research, 73(13), 3997-4008 (2013-05-08)
The transcriptional coactivator SRC-3 plays a key role in enhancing prostate cancer cell proliferation. Although SRC-3 is highly expressed in advanced prostate cancer, its role in castration-resistant prostate cancer (CRPC) driven by PTEN mutation is unknown. We documented elevated SRC-3
Brandon Faubert et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(7), 2554-2559 (2014-02-20)
One of the major metabolic changes associated with cellular transformation is enhanced nutrient utilization, which supports tumor progression by fueling both energy production and providing biosynthetic intermediates for growth. The liver kinase B1 (LKB1) is a serine/threonine kinase and tumor
François Vaillant et al.
Cancer cell, 24(1), 120-129 (2013-07-13)
The prosurvival protein BCL-2 is frequently overexpressed in estrogen receptor (ER)-positive breast cancer. We have generated ER-positive primary breast tumor xenografts that recapitulate the primary tumors and demonstrate that the BH3 mimetic ABT-737 markedly improves tumor response to the antiestrogen
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