E6407

Anti-eIF2Bε antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonyme(s) : Anti-Eukaryotic translation initiation factor 2B, ε, Anti-Eukaryotic translation initiation factor 2B, subunit 5, Anti-eIF-2B GDP-GTP exchange factor subunit epsilon
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: IgG fraction of antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~80 kDa
• Espèces réactives: mouse, rat, human
• Technique(s): indirect immunofluorescence: 1:500-1:1,000 using paraformaldehyde fixed NIH-3T3 cells.; indirect immunofluorescence: suitable; western blot: 1:500-1:1,000 using Rat1 or HEK-293T cell lysates.
• Numéro d'accès UniProt: Q13144
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... EIF2B5(8893) ; mouse ... Eif2b5(224045) ; rat ... Eif2b5(192234)

Description

Immunogène

synthetic peptide corresponding to amino acids 50-65 of human eIF2ε conjugated to KLH. The corresponding sequence is identical in rat and mouse.

Application

Anti-eIF2Bε antibody produced in rabbit is suitable for indirect immunofluorescence (at a dilution of 1:500-1:1,000 using paraformaldehyde fixed NIH-3T3 cells) and western blotting at a dilution of 1:500-1:1,000 using Rat1 or HEK-293T cell lysates.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Actions biochimiques/physiologiques

Translation initiation factor eIF-2B subunit ε is a protein encoded by the EIF2B5 gene in humans. The eIF2B is composed of five subunits α, β, γ, δ and ε, within which the ε subunit is responsible for catalyzing the guanine exchange reaction. Defect in any of these 5 subunits leads to diseases. Mutation in the subunit of eIF2B is associated with an autosomal recessive leukoencephalopathy called Vanishing white matter (VWM).

Description de la cible

eIF2Bε encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)