GH1

89072610, rat brain, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: GH1, 89072610
• Source biologique: rat brain
• Mode de croissance: Semi-adherent
• Caryotype: Hypodiploid, modal no. 40.
• Morphologie: Epithelial
• Produits: Rat growth hormone, lactogenic hormones.
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat Wistar-Furth pituitary tumour

Description de la lignée cellulaire

Derived from pituitary gland of Wistar-Furth rats. The cells secrete growth hormone at a rate of 1μg/ml cell protein/day. Re-inoculation of cloned cells back to Wistar-Furth rats results in a marked increase in body weight and stimulation of linear growth in both intact and hypophysectomized animals independent of rat tumour size.

Application

Hormone studies

Milieu de culture

Ham′s F10 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. These cells only semi-attach and may return to suspension during transit. If this has occurred on receipt of the cells pellet cells b

Autres remarques

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