MABN318

Anti-Transmembrane Prostatic Acid Phosphatase Antibody/TMPAP, clone 3G10.1

clone 3G10.1, from mouse

• Synonyme(s) : Prostatic acid phosphatase, PAP, 5′-nucleotidase, 5′-NT, Ecto-5′-nucleotidase, Thiamine monophosphatase, Transmembrane Prostatic Acid Phosphatase, TMPase, ACPP, TMPAP
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 3G10.1, monoclonal
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_001127666
• Numéro d'accès UniProt: P15309
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ACPP(55)

Description

Description générale

ACPP, also known as Prostatic acid phosphatase, or PAP, 5′-nucleotidase (5′-NT), Ecto-5′-nuclotidase, or Thiamine monophosphatase (TMPase) , and encoded by the gene ACPP, is a non-specific tyrosine phosphatase that has both a secreted form and a cell membrane bound form. ACPP dephosphorylates a diverse number of substrates including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. ACPP also has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Additionally, the membrane bound form of ACPP has ecto-5′-nucleotidase activity in dorsal root ganglion (DRG) neurons and acts as a pain suppressor. Finally ACPP can also act as a tumor suppressor of prostate cancer. ACPP isoform 1is secreted and isoform 2 is membrane bound. Isoform 2 appears to shuttle between the cell membrane, lysosomes, and intracellular vesicles. ACPP is expressed in many tissues and is used as a marker in various stages of prostatic cancer diagnostics. Also the enzyme was intensely studied in HIV infection because it can be proteolytically cleaved in seminal fluid to produce several peptides (SEVI peptides) one of which, PAPf39, happens to enhance HIV infection by binding HIV virions and helping to attach them to target cells--interestingly such SEVI peptides are degraded in vitro by EGCG, a compound found in green tea extract.

Spécificité

This antibody is expected to cross react with isoform 2 based upon immunogen design.

Immunogène

GST-tagged recombinant protein corresponding to human Transmembrane Prostatic Acid Phosphatase/TMPAP.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Transmembrane Prostatic Acid Phosphatase/TMPAP in human prostate tissue.

Research Category
Neuroscience

This Anti-Transmembrane Prostatic Acid Phosphatase Antibody/TMPAP, clone 3G10.1 is validated for use in western blotting & IHC (Paraffin) for the detection of Transmembrane Prostatic Acid Phosphatase/TMPAP.

Qualité

Evaluated by Western Blotting in human prostate tumor tissue lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected this antibody detected Transmembrane Prostatic Acid Phosphatase/TMPAP in 200 µg of human prostate tumor tisssue lysate.

Description de la cible

~48 kDa observed. Two isoforms are known to exist at 45 kDa (isoform 1) and 48 kDa (isoform 2). Uncharacterized band(s) may be observed in some cell lysates.

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable