MAB3238
Anti-Cytokeratin 19 Antibody, clone RCK108
clone RCK108, Chemicon®, from mouse
Synonyme(s) :
Anti-CK19, Anti-K19, Anti-K1CS
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
RCK108, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Chemicon®
Technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... KRT19(3880)
Description générale
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
Cytokeratin 19 (molecular mass 40 kD) is a marker of simple epithelia. Cytokeratin 19 has been found in mesothelial and mesothelioma cells, and in ovarian cysts, cystadenomas, and ovarian carcinomas. It has been shown to be present in the basal layer of non-keratinizing stratified squamous epithelia such as the oral cavity and the ectocervix. Cytokeratin 19 has also been found in adenocarcinomas of the lung and in tumor cells of pulmonary metastases.
It has been reported that cytokeratin 19 can be found in the ductal cells of normal pancreas and in pancreas cancers. It was found that ductal cells of normal pancreas express a number of cytokeratins including cytokeratin 19, whereas acinar cells of normal pancreas also express numerous cytokeratins but not cytokeratin 19. Thus it is determined that pancreas cancers consistently express certain cytokeratins including cytokeratin 19, as do normal ductal cells, which indicates that pancreas cancers may originate from normal ductal cells.
Cytokeratin 19 (molecular mass 40 kD) is a marker of simple epithelia. Cytokeratin 19 has been found in mesothelial and mesothelioma cells, and in ovarian cysts, cystadenomas, and ovarian carcinomas. It has been shown to be present in the basal layer of non-keratinizing stratified squamous epithelia such as the oral cavity and the ectocervix. Cytokeratin 19 has also been found in adenocarcinomas of the lung and in tumor cells of pulmonary metastases.
It has been reported that cytokeratin 19 can be found in the ductal cells of normal pancreas and in pancreas cancers. It was found that ductal cells of normal pancreas express a number of cytokeratins including cytokeratin 19, whereas acinar cells of normal pancreas also express numerous cytokeratins but not cytokeratin 19. Thus it is determined that pancreas cancers consistently express certain cytokeratins including cytokeratin 19, as do normal ductal cells, which indicates that pancreas cancers may originate from normal ductal cells.
Spécificité
Cytokeratin 19. MAB3238 reacts with glandular epithelial cells and most carcinomas. Does not react with hepatocytes and hepatocarcinoma
Immunogène
Cytoskeletal preparation of T24 cells.
Application
Anti-Cytokeratin 19 Antibody, clone RCK108 is an antibody against Cytokeratin 19 for use in FC, WB, IC, IH(P).
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytokeratins
Cytokeratins
Western blot: 1:100-1:1,000; Recognizes a 40kDa band in reduced westerns
Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissue (after treatment with pepsin or trypsin) sections.
Immunocytochemistry: 1:100-1:200
Flow cytometry: 1:100-1:200.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissue (after treatment with pepsin or trypsin) sections.
Immunocytochemistry: 1:100-1:200
Flow cytometry: 1:100-1:200.
Optimal working dilutions must be determined by the end user.
Forme physique
Format: Purified
Liquid in buffer with 0.1% sodium azide.
Stockage et stabilité
Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Ji-Eun Seo et al.
ALTEX, 39(4), 583-604 (2022-07-07)
Three-dimensional (3D) culture systems are increasingly being used for genotoxicity studies due to improved cell-to-cell interactions and tissue-like structures that are limited or lacking in 2D cultures. The present study optimized a 3D culture system using metabolically
Reduced keratin expression in colorectal neoplasia and associated fields is reversible by diet and resection.
Evans, CA; Rosser, R; Waby, JS; Noirel, J; Lai, D; Wright, PC; Williams, EA; Riley et al.
BMJ Open Gastroenterology null
Natasha P Clayton et al.
Toxicologic pathology, 46(6), 653-659 (2018-08-10)
The use of three-dimensional (3-D) in vitro culture systems (spheroids, organoids) in biomolecular and drug discovery research has become increasingly popular. The popularity is due, in part, to a diminished reliance on animal bioassays and a desire to develop physiologically
A Unique Expression of Keratin 14 in a Subset of Trophoblast Cells.
Abou-Kheir, W; Eid, A; El-Merahbi, R; Assaf, R; Daoud, G
Testing null
Luca Pontiggia et al.
The Journal of investigative dermatology, 134(6), 1735-1742 (2014-01-23)
In our previous work, we showed that human sweat gland-derived epithelial cells represent an alternative source of keratinocytes to grow a near normal autologous epidermis. The role of subtypes of sweat gland cells in epidermal regeneration and maintenance remained unclear.
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