ABE1949
Anti-RLIM/Rnf12 Antibody
serum, from rabbit
Synonyme(s) :
Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, RLIM/Rnf12
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
serum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
hamster, human, mouse
Technique(s)
ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... RLIM(51132)
Description générale
E3 ubiquitin-protein ligase RLIM (UniProt Q9WTV7; also known as Hoxa1 regulated, LIM domain-interacting RING finger protein, R-LIM, RING finger LIM domain-binding protein, Ring finger protein 12) is encoded by the Rlim (also known as AL022832, AW743871, Ha1r, Rlim1, Rnf12) gene (Gene ID 19820) in murine species. The CLIM/LDB/NLI and RLIM/RNF12 cofactors are transcriptional coregulatory proteins characterized by their ability to bind the LIM domains of nuclear LIM proteins, including LIM homeodomain (LIM-HD) transcription factors. The association of CLIM with LIM-HDs positively regulate their biological and transcriptional activity. On the other hand, the RING finger ubiquitin ligase RLIM acts as a negative regulator of LIM-HD activity by interacting with and targeting CLIM for degradation via the ubiquitin-proteasome pathway.
Spécificité
Exhibits no cross-reactivity toward the homologous ubiquitin ligase Rnf6.
Immunogène
Recombinant protein corresponding to mouse RLIM/Rnf12.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
This Anti-RLIM/Rnf12 Antibody is validated for use in Western Blotting, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunofluorescence for the detection of RLIM/Rnf12.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot selectively detected endogenouse RLIM in HEK293 lysate and in vitro transcribed and translated (TNT) RLIM, but not TNT Rnf6.(Courtesy of Dr. Ingolf Bach, University of Massachusetts Medical School).
Western Blotting Analysis: A representative lots detected RLIM in cell and tissue lysates of human, murine, and Chinese hamster species (Jiao, B., et al. (2013). Mol Biol Cell. 24(19):3085-3096; Jiao, B., et al. (2012). Cell. 149(3):630-641; Shin, J., et al. (2010). Nature. 467(7318):977-981; Gungor, C., et al. (2007). Proc. Natl. Acad. Sci. USA. 104(38):15000-15005; Ostendorff, H.P., et al. (2006). Dev Dyn. 235(3):786-791; Kramer, O.H., et al. (2003). EMBO. 22(13):3411-3420; Ostendorff, H.P., et al. (2002). Nature. 416(6876):99-103).
Immunocytochemistry Analysis: A representative lots detected RLIM in cultured murine mammary epithelial cells and MEFs by fluorescent immunocytochemistry (Jiao, B., et al. (2012). Cell. 149(3):630-641; Shin, J., et al. (2010). Nature. 467(7318):977-981).
Immunocytochemistry Analysis: A representative lot detected predominant nuclear expression of RLIM in HeLa, MCF-7, HEK293, and murine pituitary gonadotroph Alpha T3 cells by fluorescent immunocytochemistry (Jiao, B., et al. (2013). Mol Biol Cell. 24(19):3085-3096).
Chromatin Immunoprecipitation Analysis: A representative lots detected RLIM association at the alphaGSU promoter in murine pituitary gonadotroph Alpha T3 cells, as well as estrogen-induced RLIM recruitment to the estrogen response element (ERE) in the promoter region of PS2 and WISP2 in MCF-7 cells (Ostendorff, H.P., et al. (2002). Nature. 416(6876):99-103; Johnsen, S.A., et al. (2009). Cancer Res. 69(1):128-136).
Immunohistochemistry Analysis: A representative lot detected similar stage-dependent expression patterns between RLIM and CLIM1/2 in the developing neural tube during early mouse embryogenesis (Ostendorff, H.P., et al. (2006). Dev Dyn. 235(3):786-791).
Immunohistochemistry Analysis: A representative lot detected a RLIM expression pattern distinct from that of the homologous ubiquitin ligase Rnf6 in E12.5-E13 murine embryonic spinal cord (Tursun, B., et al. (2005). Genes Dev. 19(19):2307-2319).
Immunohistochemistry Analysis: A representative lot detected RLIM-positive cells in breast epidermis and oral cavity epithelium using paraffin-embedded adult human tissue sections (Jiao, B., et al. (2013). Mol Biol Cell. 24(19):3085-3096).
Immunohistochemistry Analysis: A representative lot detected RLIM accumulation during oocyte maturation using paraffin-embedded ovary sections from female mice (Shin, J., et al. (2010). Nature. 467(7318):977-981).
Immunofluorescence Analysis: A representative lot detected RLIM nuclear co-localization with ERalpha in frozen human breast tumor sections by dual fluorescent immunohistochemistry (Johnsen, S.A., et al. (2009). Cancer Res. 69(1):128-136).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated CLIM and ERalpha with RLIM from MCF-7 lysates (Johnsen, S.A., et al. (2009). Cancer Res. 69(1):128-136).
Immunofluorescence Analysis: A representative lot detected RLIM nuclear co-localization with H3K27me3 immunoreactivity in paraffin-embedded E7-7.5 mouse embryos by dual fluorescent immunohistochemistry (Shin, J., et al. (2014). Nature. 511(7507):86-89).
Western Blotting Analysis: A representative lots detected RLIM in cell and tissue lysates of human, murine, and Chinese hamster species (Jiao, B., et al. (2013). Mol Biol Cell. 24(19):3085-3096; Jiao, B., et al. (2012). Cell. 149(3):630-641; Shin, J., et al. (2010). Nature. 467(7318):977-981; Gungor, C., et al. (2007). Proc. Natl. Acad. Sci. USA. 104(38):15000-15005; Ostendorff, H.P., et al. (2006). Dev Dyn. 235(3):786-791; Kramer, O.H., et al. (2003). EMBO. 22(13):3411-3420; Ostendorff, H.P., et al. (2002). Nature. 416(6876):99-103).
Immunocytochemistry Analysis: A representative lots detected RLIM in cultured murine mammary epithelial cells and MEFs by fluorescent immunocytochemistry (Jiao, B., et al. (2012). Cell. 149(3):630-641; Shin, J., et al. (2010). Nature. 467(7318):977-981).
Immunocytochemistry Analysis: A representative lot detected predominant nuclear expression of RLIM in HeLa, MCF-7, HEK293, and murine pituitary gonadotroph Alpha T3 cells by fluorescent immunocytochemistry (Jiao, B., et al. (2013). Mol Biol Cell. 24(19):3085-3096).
Chromatin Immunoprecipitation Analysis: A representative lots detected RLIM association at the alphaGSU promoter in murine pituitary gonadotroph Alpha T3 cells, as well as estrogen-induced RLIM recruitment to the estrogen response element (ERE) in the promoter region of PS2 and WISP2 in MCF-7 cells (Ostendorff, H.P., et al. (2002). Nature. 416(6876):99-103; Johnsen, S.A., et al. (2009). Cancer Res. 69(1):128-136).
Immunohistochemistry Analysis: A representative lot detected similar stage-dependent expression patterns between RLIM and CLIM1/2 in the developing neural tube during early mouse embryogenesis (Ostendorff, H.P., et al. (2006). Dev Dyn. 235(3):786-791).
Immunohistochemistry Analysis: A representative lot detected a RLIM expression pattern distinct from that of the homologous ubiquitin ligase Rnf6 in E12.5-E13 murine embryonic spinal cord (Tursun, B., et al. (2005). Genes Dev. 19(19):2307-2319).
Immunohistochemistry Analysis: A representative lot detected RLIM-positive cells in breast epidermis and oral cavity epithelium using paraffin-embedded adult human tissue sections (Jiao, B., et al. (2013). Mol Biol Cell. 24(19):3085-3096).
Immunohistochemistry Analysis: A representative lot detected RLIM accumulation during oocyte maturation using paraffin-embedded ovary sections from female mice (Shin, J., et al. (2010). Nature. 467(7318):977-981).
Immunofluorescence Analysis: A representative lot detected RLIM nuclear co-localization with ERalpha in frozen human breast tumor sections by dual fluorescent immunohistochemistry (Johnsen, S.A., et al. (2009). Cancer Res. 69(1):128-136).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated CLIM and ERalpha with RLIM from MCF-7 lysates (Johnsen, S.A., et al. (2009). Cancer Res. 69(1):128-136).
Immunofluorescence Analysis: A representative lot detected RLIM nuclear co-localization with H3K27me3 immunoreactivity in paraffin-embedded E7-7.5 mouse embryos by dual fluorescent immunohistochemistry (Shin, J., et al. (2014). Nature. 511(7507):86-89).
Qualité
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: A 1:5,000 of this antibody detected RLIM/Rnf12 in 10 µg of mouse brain tissue lysate.
Western Blotting Analysis: A 1:5,000 of this antibody detected RLIM/Rnf12 in 10 µg of mouse brain tissue lysate.
Description de la cible
~69 kDa observed. Uncharacterized band(s) may appear in some lysates.
Forme physique
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Elsa J Sousa et al.
Cell stem cell, 22(6), 919-928 (2018-05-29)
A hallmark of naive pluripotency is the presence of two active X chromosomes in females. It is not clear whether prevention of X chromosome inactivation (XCI) is mediated by gene networks that preserve the naive state. Here, we show that
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique