ABC1688

Anti-SWAP-70

from rabbit, purified by affinity chromatography

• Synonyme(s) : Switch-associated protein 70
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Numéro d'accès NCBI: NP_001284643
• Numéro d'accès UniProt: Q9UH65
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SWAP70(23075)

Description

Description générale

Switch-associated protein 70 (UniProt: Q9UH65; also known as SWAP-70) is encoded by the SWAP70 (also known as KIAA0640, HSPC321) gene (Gene ID: 23075) in human. SWAP-70 is a phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent guanine nucleotide exchange factor (GEF) that, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. SWAP-70 regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion. It is expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue. In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG, but not IgM. It is also widely expressed in spleen, kidney, lung, and liver. SWAP-70 has an N-terminal putative EF-hand domain, a pleckstrin homology (PH) domain (aa 210-306) that binds phosphatidylinositol 3,4,5-trisphosphate and is responsible for membrane localization, a tri-partite coiled-coil region (aa 316-539), and a C-terminal F-actin-binding site through which it specifically binds non-muscle actin. (Ref.: Chacón-Martínez, CA et al. (2103). J. Biol. Chem. 288(40); 28687-28703).

Spécificité

This rabbit polyclonal antibody detects human Switch-associated protein 70. It targets an epitope with in 12 amino acids from the N-terminal region.

Immunogène

KLH-conjugated linear peptide corresponding to 12 amino acids from the N-terminal region of human Switch-associated protein 70.

Application

Anti-SWAP-70, Cat. No. ABC1688, is a rabbit polyclonal antibody that detects Switch-associated protein 70 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected SWAP-70 in human tonsil and human lung tissue sections.

Qualité

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected SWAP-70 in Raji cell lysate.

Description de la cible

~70 kDa observed; 69.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable