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Documents

COA/CoQ

04-841

Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal

Name: Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal
Brand: MM

culture supernatant, clone A9W4, from rabbit

Synonyme(s) :

RhoA-binding kinase 2, Rho-associated, coiled-coil containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, p150 ROK-alpha, p164 ROCK-2, ROCK-II, ROKalpha, Rock2

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About This Item

Code UNSPSC :

12352203

eCl@ss :

32160702

Nomenclature NACRES :

NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

culture supernatant

Type de produit anticorps

primary antibodies

Clone

A9W4, monoclonal

Espèces réactives

rat, human, mouse, bovine

Technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

Numéro d'accès NCBI

NM_013022

Numéro d'accès UniProt

Q62868

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

rat ... Rock2(25537)

Description générale

Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.

Spécificité

Predicted to cross-react with mouse and bovine based on sequence homology.

Recognizes ROKα/ROCK-II, Mr 150 kDa. Does not recognize ROKβ/ROCK-I. A non-specific band may also be detected, Mr 80 kDa.

Immunogène

6His-tagged fusion protein corresponding to residues 2-543 of rat ROKα/ROCK-II.

Application

Detect ROKα/ROCK-II using this Anti-ROKα/ROCK-II Antibody, clone A9W4 validated for use in IP & WB.

Immunoprecipitation:
4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Qualité

Routinely evaluated by Western BLot on RIPA lysates from PC-12 cells.

Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells.

Description de la cible

150 kDa

Liaison

Replaces: 05-841

Forme physique

Cultured supernatant containing 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
RIPA lysates from PC-12 cells

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton.

T Leung et al.

Molecular and cellular biology, 16(10), 5313-5327 (1996-10-01)

The GTPase RhoA has been implicated in various cellular activities, including the formation of stress fibers, motility, and cytokinesis. We recently reported on a p150 serine/threonine kinase (termed ROK alpha) binding RhoA only in its active GTP-bound state and on

The PRK2 kinase is a potential effector target of both Rho and Rac GTPases and regulates actin cytoskeletal organization.

S Vincent et al.

Molecular and cellular biology, 17(4), 2247-2256 (1997-04-01)

The Ras-related Rho family GTPases mediate signal transduction pathways that regulate a variety of cellular processes. Like Ras, the Rho proteins (which include Rho, Rac, and CDC42) interact directly with protein kinases, which are likely to serve as downstream effector

A cell-autonomous tumour suppressor role of RAF1 in hepatocarcinogenesis.

Ines Jeric et al.

Nature communications, 7, 13781-13781 (2016-12-22)

Hepatocellular carcinoma (HCC) is a leading cause of cancer deaths, but its molecular heterogeneity hampers the design of targeted therapies. Currently, the only therapeutic option for advanced HCC is Sorafenib, an inhibitor whose targets include RAF. Unexpectedly, RAF1 expression is

ROCK1 and 2 differentially regulate actomyosin organization to drive cell and synaptic polarity.

Karen A Newell-Litwa et al.

The Journal of cell biology, 210(2), 225-242 (2015-07-15)

RhoGTPases organize the actin cytoskeleton to generate diverse polarities, from front-back polarity in migrating cells to dendritic spine morphology in neurons. For example, RhoA through its effector kinase, RhoA kinase (ROCK), activates myosin II to form actomyosin filament bundles and

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