CRISPR Essentials: MISSION® CRISPR gRNAs, Cas9 and Related Products
Compare and contrast the features of CRISPR ribonucleoprotein (RNP) products: guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments including knockout/knock-in, activation, and inhibition. Select the best formats for your specific applications from synthetic gRNA, plasmid DNA, lentiviral particles, and proteins.
Section Overview
CRISPR RNP
Single-guide Synthetic RNA
Synthetic CRISPR gRNAs with guaranteed activity
Two-part Synthetic gRNA |
|---|
CRISPR two-part system requires both crRNA and tracrRNA to complex with Cas9.
Cas9 Proteins |
|---|
mRNA, PLASMIDS, AND LENTIVIRUS
Sanger QuickPick™ Knockout Pre-cloned Guides |
|---|
Cas9 Products |
|---|
CRISPR Controls
Negative Controls |
|---|
Positive Controls |
|---|
NON-MAMMALIAN CRISPR SYSTEMS
Plant CRISPR
Explore ready-to-use cas9 and guide RNA (gRNA) expression plasmids for monocots and dicots
- Our plant CRISPR/Cas9 products are intended for Agrobacterium-mediated plant transformation or biolistic microparticle bombardment or protoplast transformation
- A codon optimized Cas9 protein and a gRNA are expressed from a single vector and provided as ready-to-use, transfection-grade DNA.
Bacterial CRISPR
Our E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either ssDNA or dsDNA as an editing template.
Vectors
Mammalian System Vectors |
|---|
Lentiviral Vectors |
|---|
Plant Vectors |
|---|
Bacterial Vectors |
|---|
Vector Maps and Sequences
Click on the images below for a larger view.
If you need any further information, please contact us here and a customer service representative will get back to you.
Product Guarantee
We are so confident in the performance of our synthetic gRNA products, that we fully guarantee the quality and performance of any gRNA we produce, including custom sequences. If your crRNA or sgRNA do not yield detectable cleavage at the intended target site, we will provide you a one-time replacement, free of charge.
To qualify for this guarantee, please send an image or sequencing data from a single experiment demonstrating detectable cleavage using one of our positive controls, side-by-side with the negative results from your synthetic gRNA. To receive your replacement, simply email oligotechserv@milliporesigma.com and include sample data from a representative experiment (T7E1, TIDE, or NGS).
To continue reading please sign in or create an account.
Don't Have An Account?