HPLC Analysis of Proglumide Enantiomers on Astec^® Cellulose DMP

column

Astec Cellulose DMP, 15 cm x 4.6 mm I.D., 5 μm particles (51098AST)

mobile phase

[A] heptane; [B] IPA; [C] TFA; (90:10:0.1, A:B:C)

flow rate

0.5 mL/min

column temp.

25 °C

detector

UV, 254 nm

injection

2 μL

sample

2 mg/mL in mobile phase