HPLC Analysis of mAb 321 on Proteomix® SCX NP5 versus Competitive SCX using pH and Salt Gradients
Materials
analytical column
Product No.
Description
Pricing
Proteomix® SCX-NP5 Strong Cation Exchange Column
L × I.D. 25 cm × 4.6 mm, 5 μm particle size, PEEKCONDITIONS
column
Proteomix SCX-NP5, 25 cm x 4.6 mm I.D. (PEEK hardware), 5 μm particles (Z777164)
mobile phase
(A) 2.4 mM Tris, 1.5 mM imidazole, 11.6 mM piperazine, pH 6.0; (B) A + 0.5 M NaCl, pH 10.5
flow rate
0.8 mL/min
detector
UV, 280 nm
injection
20 μL
sample
MAb 321 (5 mg/mL)
Description
Legal Information
Proteomix is a registered trademark of Sepax Technologies