Skip to Content

Determination of polyamines in hydrolysates of uremic plasma by high-performance cation-exchange column chromatography.

Determination of polyamines in hydrolysates of uremic plasma by high-performance cation-exchange column chromatography.

Journal of chromatography (1983-02-11)

T Takagi, T G Chung, A Saito

ABSTRACT

The levels of putrescine, cadaverine, spermidine and spermine in uremic plasma were determined with an automatic polyamine analyzer with a 7.5 X 0.2 cm I.D. cation-exchange column using a stepwise sodium chloride gradient. All four polyamines were higher in ten patients with chronic renal failure than in eight normal subjects. The total polyamine content was also measured in the patients' plasma before and after maintenance dialysis; putrescine and spermidine levels were significantly lowered by the procedure.

MATERIALS

Product Number

Brand

Product Description

Supelco

Ethanol-500, 500 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

Supelco

Acetone, Pharmaceutical Secondary Standard; Certified Reference Material

Sigma-Aldrich

Trimethylamine solution, 43.0-49.0% in H₂O (T)

Supelco

Acetone, analytical standard

Sigma-Aldrich

Cadaverine, purum, ≥97.0% (GC)

Sigma-Aldrich

Zinc, shot, <12 mm, 99.99% trace metals basis

Sigma-Aldrich

Zinc, pieces, 2-14 mesh, 99.9% trace metals basis

Sigma-Aldrich

Reagent Alcohol, anhydrous, ≤0.003% water

Sigma-Aldrich

Acetone, ≥99%, meets FCC analytical specifications

Sigma-Aldrich

Zinc preparation, 5 g/dL Zn⁺² in THF, highly reactive Rieke^®metal

Sigma-Aldrich

Acetone, natural, ≥97%

Sigma-Aldrich

Trimethylamine solution, 25 wt. % in propylene glycol

Sigma-Aldrich

Zinc, foil, thickness 1.0 mm, 99.99% trace metals basis

Sigma-Aldrich

Zinc, granular, 20-30 mesh, ACS reagent, ≥99.8%

Supelco

Ethanol solution, certified reference material, 2000 μg/mL in methanol

Sigma-Aldrich

Trimethylamine solution, 25 wt. % in H₂O

Sigma-Aldrich

Acetone, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%

Sigma-Aldrich

Zinc, sticks, diam. 7-10 mm, 99.97% trace metals basis

Sigma-Aldrich

Zinc, nanopowder, 40-60 nm avg. part. size, ≥99% trace metals basis

Sigma-Aldrich

Zinc, foil, thickness 0.25 mm, 99.999% trace metals basis

Sigma-Aldrich

Cadaverine, 95%

Sigma-Aldrich

Zinc, shot, 5 mm, 99.999% trace metals basis

Sigma-Aldrich

Zinc, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.9%, granular

Sigma-Aldrich

Zinc, dust, <10 μm, ≥98%

Sigma-Aldrich

Acetone, histological grade, ≥99.5%

Sigma-Aldrich

Acetone, ACS reagent, ≥99.5%

Sigma-Aldrich

Acetone, ACS reagent, ≥99.5%

Sigma-Aldrich

Acetone, suitable for HPLC, ≥99.9%

Supelco

Ethanol-150, 150 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

Supelco

Ethanol-80, 80 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®