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Markers of dopamine metabolism in Parkinson's disease. The Parkinson Study Group.

Markers of dopamine metabolism in Parkinson's disease. The Parkinson Study Group.

Neurology (1992-11-01)

P A LeWitt, M P Galloway, W Matson, P Milbury, M McDermott, D K Srivastava, D Oakes

ABSTRACT

We used two analytic methods (a multichannel coulometric electrode array with high-performance liquid chromatography, and gas chromatography-mass spectrophotometry) to measure CSF dopamine (DA) and its metabolites in mildly affected, unmedicated subjects with Parkinson's disease (PD). The mean (+/- SD) concentration of homovanillic acid (HVA), the most abundant product of DA turnover, was 164.57 +/- 95.05 nM. As sequential aliquots of CSF were collected from the first to 23rd ml, CSF HVA concentration almost doubled. After HVA, 3-O-methyldopa (3-O-MD) was the next most abundant compound. The summed concentrations of 3-O-MD, 3,4-dihydroxyphenylacetic acid, 3-methoxytyramine, DA, DA-3-sulfate, homovanillol, and levodopa (LD) amounted to 12.6% of HVA. Concentrations of the DA metabolites did not correlate to a variety of indices of PD severity. The presence of LD and 3-O-MD may be indicators of DA synthesis and possibly could reflect compensatory processes among surviving dopaminergic neurons of the PD brain.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Nickel, powder, <50 μm, 99.7% trace metals basis

Sigma-Aldrich

Zirconium, powder, −100 mesh

Sigma-Aldrich

Copper, powder, <75 μm, 99%

Sigma-Aldrich

Magnesium, rod, diam. 6 mm, ≥99.9% trace metals basis

Sigma-Aldrich

Titanium, foil, thickness 0.025 mm, 99.98% trace metals basis

Sigma-Aldrich

Zinc, dust, <10 μm, ≥98%

Sigma-Aldrich

Copper, beads, 2-8 mm, 99.9995% trace metals basis

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Molybdenum, foil, thickness 0.025 mm, ≥99.9% trace metals basis

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Vanadium, foil, thickness 0.127 mm, 99.7% trace metals basis

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Nickel, rod, diam. 6.35 mm, ≥99.99% trace metals basis

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Titanium, wire, diam. 1.0 mm, 99.99% trace metals basis

Sigma-Aldrich

Nickel, foil, thickness 0.5 mm, 99.98% trace metals basis

Sigma-Aldrich

Copper, foil, thickness 0.5 mm, 99.98% trace metals basis

Sigma-Aldrich

Zinc preparation, 5 g/dL Zn⁺² in THF, highly reactive Rieke^®metal

Sigma-Aldrich

Nickel, wire, diam. 0.5 mm, ≥99.9% trace metals basis

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Copper, wire, diam. 0.64 mm, 99.995% trace metals basis

Sigma-Aldrich

Copper, wire, diam. 1.0 mm, ≥99.9%

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Manganese, chips, thickness <2.0 mm, 99%

Sigma-Aldrich

Copper, wire, diam. 2.0 mm, 99.999% trace metals basis

Sigma-Aldrich

Magnesium, dendritic pieces, purified by distillation, 99.998% trace metals basis

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Magnesium, turnings, reagent grade, 98%

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Cobalt, foil, thickness 0.1 mm, ≥99.99%

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Aluminum, wire, diam. 0.58 mm, ≥99.99% trace metals basis

Sigma-Aldrich

Aluminum, pellets, 3-8 mesh, ≥99.999% trace metals basis

Sigma-Aldrich

Aluminum, wire, diam. 1.0 mm, 99.999% trace metals basis

Sigma-Aldrich

Aluminum, granular, <1 mm, 99.7% trace metals basis

Sigma-Aldrich

Tin, nanopowder, <150 nm particle size (SEM), ≥99% trace metals basis

Titanium, IRMM^®, certified reference material, 0.5 mm wire

Aluminum, IRMM^®, certified reference material, 1.0 mm wire

Titanium, IRMM^®, certified reference material, 0.5 mm foil