Prostaglandin E1 transport into rat intestinal cryptic cell line IEC-6.

Prostaglandin E1 transport into rat intestinal cryptic cell line IEC-6.

Research communications in molecular pathology and pharmacology (2008-04-23)

Kohji Hirata, Kumiko Mabashi, Toshiharu Horie

ABSTRACT

Administration of antitumor drugs such as 5-fluorouracil and methotrexate to rats causes intestinal injury. The intestinal injury was reported to be prevented by coadministration of prostaglandin E1 analogues to rats. IEC-6 cell is a useful in vitro model to study the cytoprotective effect in enterocytes, For its purpose, it is important to know whether or not prostaglandins are taken up into IEC-6 cells. The transport of prostaglandin E1 into IEC-6 cells was investigated, using [3H]-prostaglandin E1. Prostaglandin E1 was found to be transported into IEC-6 cells by the passive diffusion mechanism.

MATERIALS

Product Number

Brand

Product Description

114359

Sigma-Aldrich

Bromocresol Green, ACS reagent, Dye content 95 %

17470

Sigma-Aldrich

Bromocresol Green Sultone Form, for microscopy (Bot., Hist., Vit.), indicator (pH 3.8-5.4)