Skip to Content
Merck
  • IQGAP1 is necessary for pulmonary vascular barrier protection in murine acute lung injury and pneumonia.

IQGAP1 is necessary for pulmonary vascular barrier protection in murine acute lung injury and pneumonia.

American journal of physiology. Lung cellular and molecular physiology (2012-05-09)
M Bhattacharya, G Su, X Su, J A Oses-Prieto, J T Li, X Huang, H Hernandez, A Atakilit, A L Burlingame, M A Matthay, D Sheppard
ABSTRACT

We recently reported that integrin α(v)β(3) is necessary for vascular barrier protection in mouse models of acute lung injury and peritonitis. Here, we used mass spectrometric sequencing of integrin complexes to isolate the novel β(3)-integrin binding partner IQGAP1. Like integrin β(3), IQGAP1 localized to the endothelial cell-cell junction after sphingosine-1-phosphate (S1P) treatment, and IQGAP1 knockdown prevented cortical actin formation and barrier enhancement in response to S1P. Furthermore, knockdown of IQGAP1 prevented localization of integrin α(v)β(3) to the cell-cell junction. Similar to β(3)-null animals, IQGAP1-null mice had increased pulmonary vascular leak compared with wild-type controls 3 days after intratracheal LPS. In an Escherichia coli pneumonia model, IQGAP1 knockout mice had increased lung weights, lung water, and lung extravascular plasma equivalents of (125)I-labeled albumin compared with wild-type controls. Taken together, these experiments indicate that IQGAP1 is necessary for S1P-mediated vascular barrier protection during acute lung injury and is required for junctional localization of the barrier-protective integrin α(v)β(3).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-IQGAP1 Antibody, clone AF4, clone AF4, Upstate®, from mouse
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 μg
1 Estimated to ship on 14 January 2025
Details...
€512.00