F4772

Monoclonal Anti-Cytokeratin Peptide 18−FITC antibody produced in mouse

clone CY-90, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Monoclonal Anti-Cytokeratin Peptide 18
• MDL number: MFCD00162250
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: CY-90, monoclonal
• form: buffered aqueous solution
• species reactivity: wide range
• technique(s): direct immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded sections of human or animal tissues; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... KRT18(3875) , KRT18(3875)

Description

General description

Broadly keratins are grouped into two types on the basis of their biochemical and immunological properties: Type II keratins are a family of basic proteins identified as K1 to K8, whereas type I keratins consists of K9 to K20, belong to the acidic group. It belongs to type I acrylic sub-family and is differentially expressed in various human tissues.

FITC Monoclonal Anti-Cytokeratin Peptide 18 is a purified mouse monoclonal antibody conjugated with fluorescein isothiocyanate (FITC) isomer I. Monoclonal Anti-Cytokeratin Peptide 18 (mouse IgG1 isotype) is derived from the CY-90 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice. Cytokeratin 18 (CYK18) is a 45 kDa polypeptide, differentially expressed in various human tissues.

Immunogen

human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.

Application

Monoclonal Anti-Cytokeratin Peptide 18 FITC antibody produced in mouse has been used in:

• direct immunofluorescent staining
• immunohistochemistry
• double immunofluorescence
• immunocytochemical and immuno?electron microscopic localization of keratins

Monoclonal Anti-Cytokeratin Peptide 18-FITC antibody:

• is suitable for immunohistochemistry to identify renal tubular epithelial cells in the extrarenal cells regeneration during acute renal failure
• is suitable for immunocytochemical and immuno-electron microscopic localisation of keratins in human materno-foetal interaction zone
• may be used for single or double labeling procedures

Biochem/physiol Actions

Cytokeratins also called keratins (Ks) according to the new nomenclature system.These are known to be the major structural proteins of epithelial cells. It is heteropolymeric in nature i.e. in cells more than one keratin protein is always present. It belongs to type I acrylic sub-family and is differentially expressed in various human tissues. It maintains the structural integrity of extra-embryonic membrane epithelia. Broadly keratins are grouped into two types on the basis of their biochemical and immunological properties: Type II keratins are a family of basic proteins identified as K1 to K8, whereas type I keratins consists of K9 to K20, belong to the acidic group.

Monoclonal anti-cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. FITC Monoclonal Anti-Cytokeratin Peptide 18 is a chain specific antibody which can facilitate typing of normal, metaplastic and neoplastic cells. Cytokeratin 18 (CYK18) is a cytoskeletal protein associated with apoptosis. CK-18 is considered as a promising plasma biomarker to identify patients with HELLP (hemolysis, elevated liver enzymes, low platelets) syndrome.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA with 15 mM sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable