FSUPMMRO
Roche
FastStart Universal Probe Master (Rox)
sufficient for 250 reactions, sufficient for 1250 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
41106300
NACRES:
NA.55
Recommended Products
usage
sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions
Quality Level
feature
dNTPs included: no
hotstart
manufacturer/tradename
Roche
packaging
pkg of 1250 x 20 μL reactions (04913957001)
pkg of 250 x 20 μL reactions (04913949001)
pkg of 5000 x 20 μL reactions (04914058001)
technique(s)
RT-qPCR: suitable
qPCR: suitable
input
purified DNA
detection method
probe-based
Related Categories
General description
Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart™ Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can nonspecifically bind. FastStart™ Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a preincubation step at +95°C).
Universal ready-to-use hot start reaction mix for qPCR and RT-qPCR on all real-time PCR systems requiring normalization with ROX.
FastStart™ Universal Probe Master (Rox) includes a novel reference dye that enables its use on all real-time PCR instruments requiring normalization with ROX, without modification or adjustments to the specific instrument or protocol. This ready-to-use, 2x concentrated master mix contains all reagents (except primers, probe, and template) needed for running quantitative, real-time DNA-detection assays, including qPCR and two-step qRT-PCR, in the hydrolysis probe detection format. FastStart™ Universal Probe Master (Rox) generates excellent results on instruments such as the Applied Biosystems 7900 HT Fast Real-Time PCR System or the Applied Biosystems 7500 Real-Time PCR System. This product is not intended for use with the LightCycler® Instruments.
FastStart™ Universal Probe Master (Rox) includes a novel reference dye that enables its use on all real-time PCR instruments requiring normalization with ROX, without modification or adjustments to the specific instrument or protocol. This ready-to-use, 2x concentrated master mix contains all reagents (except primers, probe, and template) needed for running quantitative, real-time DNA-detection assays, including qPCR and two-step qRT-PCR, in the hydrolysis probe detection format. FastStart™ Universal Probe Master (Rox) generates excellent results on instruments such as the Applied Biosystems 7900 HT Fast Real-Time PCR System or the Applied Biosystems 7500 Real-Time PCR System. This product is not intended for use with the LightCycler® Instruments.
Application
FastStart™ Universal Probe Master (Rox) has been used:
- in TaqMan® quantitative real-time polymerase chain reaction (qRT-PCR) reactions for the quantification of endogenous miRNAs, such as MIR376B, MIR376A and MIR181A
- in reverse transcriptase (RT-PCR) to study tumor necrosis factor (TNF) expression in whole synovial tissue of undifferentiated peripheral inflammatory arthritis (UPIA) patients
- for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich by quantitativePCR
Features and Benefits
- Increase qPCR sensitivity and specificity.
- Use the master mix with any probe-based assay.
- Amplify and detect a broad range of DNA or cDNA targets.
- Visualize amplification products on agarose gels.
- Use robotic pipetting stations to set up qPCR reactions.
- Prevent false positives resulting from carryover contamination.
Components
FastStart Universal Probe Master (Rox), 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, Nucleotides (dATP, dCTP, dGTP, dUTP), and a reference dye.
Quality
Function test: Each lot is tested for performance in qPCR using three templates: a GC–rich template, an AT-rich template, and a long template (approximately 440 bp).
Other Notes
For life science research only. Not for use in diagnostic procedures
Legal Information
FastStart is a trademark of Roche
LightCycler is a registered trademark of Roche
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
The essential function for serum response factor in T-cell development reflects its specific coupling to extracellular signal-regulated kinase signaling.
Mylona A, et al.
Molecular and Cellular Biology, 31(2), 267-276 (2011)
Matthew L Hillestad et al.
Human gene therapy, 23(10), 1116-1126 (2012-07-28)
Reporter genes are important tools for assessing vector pharmacology in vivo. Although useful, current systems are limited by (1) the need to generate a new vector for each different reporter, (2) the inability to package reporter genes in small vectors
Stefano Alivernini et al.
Frontiers in medicine, 5, 186-186 (2018-07-19)
Objectives: To examine synovial tissue (ST) predictors of clinical differentiation in patients with seronegative undifferentiated peripheral inflammatory arthritis (UPIA). Methods: Fourty-two patients with IgA/IgM-Rheumatoid Factor and anti-citrullinated peptide antibodies negative UPIA, naive to Disease-Modifying Anti-Rheumatic Drugs, underwent Gray Scale (GSUS)
Kumsal Ayse Tekirdag et al.
Autophagy, 9(3), 374-385 (2013-01-17)
Macroautophagy (autophagy herein) is a cellular catabolic mechanism activated in response to stress conditions including starvation, hypoxia and misfolded protein accumulation. Abnormalities in autophagy were associated with pathologies including cancer and neurodegenerative diseases. Hence, elucidation of the signaling pathways controlling
Gozde Korkmaz et al.
PloS one, 8(12), e82556-e82556 (2013-12-21)
Autophagy is a vesicular trafficking process responsible for the degradation of long-lived, misfolded or abnormal proteins, as well as damaged or surplus organelles. Abnormalities of the autophagic activity may result in the accumulation of protein aggregates, organelle dysfunction, and autophagy
Articles
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
Related Content
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service