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  • Chimeric Peptide Species Contribute to Divergent Dipeptide Repeat Pathology in c9ALS/FTD and SCA36.

Chimeric Peptide Species Contribute to Divergent Dipeptide Repeat Pathology in c9ALS/FTD and SCA36.

Neuron (2020-05-07)
Zachary T McEachin, Tania F Gendron, Nisha Raj, María García-Murias, Anwesha Banerjee, Ryan H Purcell, Patricia J Ward, Tiffany W Todd, Megan E Merritt-Garza, Karen Jansen-West, Chadwick M Hales, Tania García-Sobrino, Beatriz Quintáns, Christopher J Holler, Georgia Taylor, Beatriz San Millán, Susana Teijeira, Toru Yamashita, Ryuichi Ohkubo, Nicholas M Boulis, Chongchong Xu, Zhexing Wen, Nathalie Streichenberger, Brent L Fogel, Thomas Kukar, Koji Abe, Dennis W Dickson, Manuel Arias, Jonathan D Glass, Jie Jiang, Malú G Tansey, María-Jesús Sobrido, Leonard Petrucelli, Wilfried Rossoll, Gary J Bassell
PMID32375063
ABSTRACT

GGGGCC hexanucleotide repeat expansions (HREs) in C9orf72 cause amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) and lead to the production of aggregating dipeptide repeat proteins (DPRs) via repeat associated non-AUG (RAN) translation. Here, we show the similar intronic GGCCTG HREs that causes spinocerebellar ataxia type 36 (SCA36) is also translated into DPRs, including poly(GP) and poly(PR). We demonstrate that poly(GP) is more abundant in SCA36 compared to c9ALS/FTD patient tissue due to canonical AUG-mediated translation from intron-retained GGCCTG repeat RNAs. However, the frequency of the antisense RAN translation product poly(PR) is comparable between c9ALS/FTD and SCA36 patient samples. Interestingly, in SCA36 patient tissue, poly(GP) exists as a soluble species, and no TDP-43 pathology is present. We show that aggregate-prone chimeric DPR (cDPR) species underlie the divergent DPR pathology between c9ALS/FTD and SCA36. These findings reveal key differences in translation, solubility, and protein aggregation of DPRs between c9ALS/FTD and SCA36.

MATERIALS
Product Number
Brand
Product Description
MABN889
Sigma-Aldrich
Anti-C9ORF72/C9RANT (poly-GA) Antibody, clone 5E9, clone 5E9, from mouse