SML3448

Propidium monoazide

≥98% (HPLC)

• Synonym(s): 3-Amino-8-azido-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridium dichloride, Propidium monoazide dichloride
• Empirical Formula (Hill Notation): C₂₇H₃₂N₆·Cl₂
• CAS Number: 91416-20-5
• Molecular Weight: 511.49
• UNSPSC Code: 12352125
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: powder
• color: red to black
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: -10 to -25°C
• SMILES string: [N-]=[N+]=NC1=CC2=C(C=C1)C3=C(C=C(C=C3)N)[N+](CCC[N+](CC)(C)CC)=C2C4=CC=CC=C4.[Cl-].[Cl-]
• InChI key: NUMTTYQOTJKZSE-UHFFFAOYSA-M

Description

Biochem/physiol Actions

Propidium derivative that allows light-induced crosslinkage to DNA for highly selective live-dead cells analysis by PCR or fluorescence microscopy.

Propidium monoazide (PMA) is a propidium derivative with an azide group that allows crosslinkage to DNA upon light exposure. PMA is only permeable to bacteria with compromised membrane integrity, making it highly selective for sick/dying/dead cells over healthy cells. PMA is typically added to cell samples (50 μM for 1-15 min in the dark) prior to cross-linking induction (650-W halogen light source), washing and DNA extraction. Due to its higher charge, PMA (+2) is less permeant to live cells and more selective than ethidium monoazide/EMA (+1) for dead cells. The stained cells can also be viewed and analyzed by fluorescence microscopy.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable