Additionally, an extensive literature search revealed an article discussing the effect of type IV collagen on fibrin assembly kinetics. The authors indicated that as the concentration of collagen increased from 0 to 100 ng/ml, the rate of fibrin assembly increased, as evidenced by an increase in the initial slope seen in Fig. 1. The article can be found at https://www.jbc.org/content/263/15/7043.full.pdf. It may be helpful to use similar guidelines.
M7403
MCDB 153 Medium
With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
Molecular, Cellular, and Development Biology 153 medium
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Pricing and availability is not currently available.
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Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)
shipped in
ambient
storage temp.
2-8°C
General description
MCDB 153 media are modifications of Ham′s nutrient mixture F-12, designed for the growth of human keratinocytes, clonal growth of chicken embryo fibroblasts, and Chinese hamster ovary (CHO) cells using low levels of fetal bovine serum protein (FBSP), extensive trace elements or no serum protein.
Application
MCDB 153 Medium has been used as a component of melanoma isolation media (MIM) to culture human non-metastatic and metastatic melanoma cells.
Quantity
Formulated to contain 17.6 grams of powder per liter of medium.
Reconstitution
Supplement with 1.18 g/L sodium bicarbonate.
also commonly purchased with this product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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What is the recommended protocol for co-culturing keratinocytes, fibroblasts, and endothelial cells using MCDB153 media?
What is the recommended protocol for co-culturing keratinocytes, fibroblasts, and endothelial cells using MCDB153 media?
1 answer-
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이 용액으로 배양한 세포를 인체에 투여하는 것이 가능한가요?
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Unless otherwise stated, this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption or application to humans or animals.
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