Skip to Content
Merck
All Photos(1)

Key Documents

C-28057

Sigma-Aldrich

Macrophage Base Medium DXF

Basal Medium, 250 ml

Synonym(s):

Macrophage Culture Medium

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

form

liquid

packaging

pkg of 250 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

Macrophage Base Medium DXF (supplements sold separately)

Application

The Macrophage Base Medium DXF is the user-customizable version of the Macrophage Generation Media DXF that does not include cytokines. It must be adequately supplemented by the user for the generation of monocyte-derived Macrophages (MDM) from freshly isolated peripheral blood monocytes or directly from PBMC as a starting material. In the latter case, the Monocyte Attachment Medium (C-28051) is also needed. Recommended for:Human Mononuclear Cells (hMNC), freshly isolatedHuman Monocytes (hMo), freshly isolated

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Quality

All lots of PromoCell Macrophage Media DXF are subjected to comprehensive quality control tests using human peripheral blood mononuclear cells. Each lot of PromoCell Macrophage Media DXF is tested for the ability to support Macrophage differentiation verified by morphological evaluation and flow cytometry analysis. Each lot of PromoCell Monocyte Attachment Medium is tested for its ability to support optimal attachment and viability of peripheral blood Monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Complete Medium supplemented with cytokines should be used within 2 weeks. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subculture Routine

Click here for more information.

Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

Protocols

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service