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  • Marked dissociation between high noradrenaline versus low noradrenaline transporter levels in human nucleus accumbens.

Marked dissociation between high noradrenaline versus low noradrenaline transporter levels in human nucleus accumbens.

Journal of neurochemistry (2007-05-09)
Junchao Tong, Oleh Hornykiewicz, Yoshiaki Furukawa, Stephen J Kish
ABSTRACT

We recently identified a noradrenaline-rich caudomedial subdivision of the human nucleus accumbens (NACS), implying a special function for noradrenaline in this basal forebrain area involved in motivation and reward. To establish whether the NACS, as would be expected, contains similarly high levels of other noradrenergic markers, we measured dopamine-beta-hydroxylase (DBH) and noradrenaline transporter in the accumbens and, for comparison, in 23 other brain regions in autopsied human brains by immunoblotting. Although the caudomedial NACS had high DBH levels similar to those in other noradrenaline-rich areas, the noradrenaline transporter concentration was low (only 11% of that in hypothalamus). Within the accumbens, transporter concentration in the caudal portion was only slightly (by 30%) higher than that in the rostral subdivisions despite sharply increasing rostrocaudal gradients of noradrenaline (15-fold) and DBH. In contrast, the rostrocaudal gradient in the accumbens for the serotonin transporter and serotonin were similar (2-fold increase). The caudomedial NACS thus appears to represent the only instance in human brain having a striking mismatch in high levels of a monoamine neurotransmitter versus low levels of its uptake transporter. This suggests that noradrenaline signalling is much less spatially and temporally restricted in the caudomedial accumbens than in other noradrenaline-rich brain areas.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Dopamine β Hydroxylase Antibody, serum, Chemicon®
Sigma-Aldrich
Anti-Dopamine β Hydroxylase Antibody, CT, Chemicon®, from rabbit