GE28-4009-90
His MultiTrap™ Fast Flow
Cytiva 28-4009-90, pack of 4 plates
Synonym(s):
His Tag Affinity Purification
About This Item
Recommended Products
material
polypropylene and polyethylene
packaging
pack of 4 plates
manufacturer/tradename
Cytiva 28-4009-90
size
127.8 mm × 85.5 mm × 30.6 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
capacity
~40 mg binding capacity(histidine-tagged protein)
suitability
suitable for bioprocess medium
General description
Application
Loading unclarified samples directly to His MultiTrap™ FF and His MultiTrap™ HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target Proteins. His MultiTrap™ FF and His MultiTrap™ HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.
Features and Benefits
- Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* Proteins.
- Increased convenience and consistency with prepacked 96-well plates.
- No filtration needed - load unclarified sample directly, increase reproducibility, and save time.
- Use His MultiTrap™ 96-well filter plates with robotic systems or manually with centrifugation or vacuum.
- Easy scale-up to HisTrap™ FF, HisPrep™ FF 16/10 or HisTrap™ HP prepacked columns. High chemical stability and high binding capacity up to 1 mg of histidine-tagged Protein per well.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
Protocols
This page shows how to perform a small-scale expression screening of histidine-tagged membrane proteins from E. coli lysates with His MultiTrap filter plates from Cytiva.
Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.
Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.
Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.
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